Ian Wark Research Institute, University of South Australia, Mawson Lakes, SA, Australia.
J Am Soc Mass Spectrom. 2010 Feb;21(2):254-60. doi: 10.1016/j.jasms.2009.10.007. Epub 2009 Oct 17.
TOF-SIMS was applied to study the cross-sectional distribution of methylene blue and papain in porous silicon layers. Elemental and molecular information were used to study their distributions in the porous region and the chemistry of their adsorption. Methylene blue (MW = 284 Da) penetrated to the base to the pores. Positive ions (SiCH(3)(+)) suggest methylene blue binds to the substrate via its methyl groups. Negative fragments (SiOSH(3)(-) and SiO(2)SCH(-)) also suggested chemisorption via O bridging of the substrate Si and methylene blue S. The larger Papain molecule (23,406 Da) distributed itself in a similar manner to methylene blue demonstrating larger molecules can be effectively incorporated into such pore structures.
TOF-SIMS 被应用于研究亚甲蓝和木瓜蛋白酶在多孔硅层中的横截面分布。元素和分子信息被用于研究它们在多孔区域的分布和吸附的化学性质。亚甲蓝（MW=284 Da）渗透到孔的底部。正离子（SiCH(3)(+))表明亚甲蓝通过其甲基与基底结合。负碎片（SiOSH(3)(-) 和 SiO(2)SCH(-)）也表明通过基质 Si 和亚甲蓝 S 的 O 桥接发生化学吸附。较大的木瓜蛋白酶分子（23406 Da）以类似于亚甲蓝的方式分布，表明较大的分子可以有效地掺入这种孔结构中。
