迈向基于聚合酶链反应检测食源耐热弯曲杆菌的国际标准：多中心协作试验中的验证
Toward an international standard for PCR-based detection of food-borne thermotolerant Campylobacters: validation in a multicenter collaborative trial.
作者信息
Lübeck P S, Cook N, Wagner M, Fach P, Hoorfar J
机构信息
Danish Veterinary Institute, DK-1790 Copenhagen V, Denmark.
出版信息
Appl Environ Microbiol. 2003 Sep;69(9):5670-2. doi: 10.1128/AEM.69.9.5670-5672.2003.
Abstract
As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C. coli, and C. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100%. The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories.
摘要
作为一项欧洲研究项目的一部分，通过一项有12个参与实验室的国际合作试验，对一种用于检测食源耐热弯曲杆菌（空肠弯曲杆菌、大肠弯曲杆菌和海鸥弯曲杆菌）的聚合酶链反应（PCR）检测方法的性能进行了评估。对来自10种目标菌株和8种非目标菌株的DNA进行了检测，结果以凝胶电泳后阳性信号的存在情况进行报告。总体涵盖率（灵敏度）为93.7%，排他率（特异性）为100%。结果表明，该检测方法可成为国际标准，并可放心地应用于微生物实验室。
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