Fermér C, Engvall E O
National Food Administration, Biology Division, SE-751 26 Uppsala, Sweden.
J Clin Microbiol. 1999 Oct;37(10):3370-3. doi: 10.1128/JCM.37.10.3370-3373.1999.
A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection and identification of thermophilic Campylobacter species.
报道了一种灵敏的聚合酶链反应(PCR)检测方法,可检测嗜热弯曲菌空肠弯曲菌、结肠弯曲菌、海鸥弯曲菌和乌普萨拉弯曲菌。此外,通过用两种限制性内切酶消化PCR产物,实现了种属鉴别。因此,本方法有潜力用于嗜热弯曲菌种属的检测和鉴定。