Kobayashi Hideki, Miura Akihiko, Hayashi Hiroko, Ogawa Torata, Endô Takayuki, Hata Eiji, Eguchi Masashi, Yamamoto Kôsi
National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.
Appl Environ Microbiol. 2003 Sep;69(9):5690-2. doi: 10.1128/AEM.69.9.5690-5692.2003.
The prevalence of eae-positive Escherichia coli (eaeEC) in Japan was examined using rectal stool samples taken from 35 calves less than 1 month old, 107 calves more than 1 to 3 months old, 88 heifers more than 3 to 6 months old, 214 heifers over 6 months old, and cows from 95 farms. Screening with eae PCR revealed the prevalence to be, with increasing age, 31.4, 8.4, 26.1, and 14.5%, respectively. Of 51 selected eaeEC strains, more than 40% were serotyped as O26, O103, O111, O145, or O157, which are frequently detected as enterohemorrhagic E. coli types. Four strains were identified as recently reported intimin types eta, iota, and kappa.
利用从35头1月龄以下的犊牛、107头1至3月龄的犊牛、88头3至6月龄的小母牛、214头6月龄以上的小母牛以及来自95个农场的奶牛采集的直肠粪便样本，对日本产肠集聚性大肠杆菌（eaeEC）的流行情况进行了检测。采用eae聚合酶链反应（PCR）进行筛查发现，随着年龄增长，流行率分别为31.4%、8.4%、26.1%和14.5%。在选取的51株eaeEC菌株中，超过40%被血清型鉴定为O26、O103、O111、O145或O157，这些血清型常被检测为肠出血性大肠杆菌类型。有4株被鉴定为最近报道的紧密素类型eta、iota和kappa。
