Kobayashi Hideki, Pohjanvirta Tarja, Pelkonen Sinikka
National Veterinary and Food Research Institute, Kuopio Regional Laboratory, Finland.
J Vet Med Sci. 2002 Nov;64(11):1071-3. doi: 10.1292/jvms.64.1071.
The role of birds as sources of Shiga toxin-and intimin-producing Escherichia coli was studied. Fecal samples from live gulls (n=86), pigeons (n=33) and broiler chickens (n=199) from 23 flocks were analyzed for stx and eae by PCR. No stx positive samples were detected. In contrast, eae E. coli were highly prevalent among gulls (40%), and was also found in pigeons (7%) and chickens (57% of the flocks contaminated). The eae positive isolates were analyzed genetically and O-serogrouped. One isolate from a pigeon was found to have stx (2f). The isolates of gulls differed from those of pigeons and chickens, and all eae E. coli isolates from birds differed from human pathogenic strains by the lack of EHEC-hlyA and bfp/EAF as well as distribution of O-serogroups. Thus, birds cannot be regarded as important carriers of zoonotic stx or eae E. coli in Finland.
研究了鸟类作为产志贺毒素和紧密黏附素大肠杆菌来源的作用。通过PCR分析了来自23个鸡群的活海鸥（n = 86）、鸽子（n = 33）和肉鸡（n = 199）的粪便样本中的stx和eae。未检测到stx阳性样本。相比之下，eae大肠杆菌在海鸥中高度流行（40%），在鸽子中也有发现（7%），在鸡中也有发现（57%的鸡群受到污染）。对eae阳性分离株进行了基因分析和O血清型分类。发现一株来自鸽子的分离株含有stx（2f）。海鸥的分离株与鸽子和鸡的分离株不同，并且所有来自鸟类的eae大肠杆菌分离株与人类致病菌株不同，因为缺乏EHEC-hlyA和bfp/EAF以及O血清型的分布。因此，在芬兰，鸟类不能被视为产志贺毒素或eae大肠杆菌的重要人畜共患病载体。
