Regional Reference Center for Mycobacteria, Piastra dei Servizi, A. O. U. Careggi, viale Morgagni 85, 50134 Florence, Italy.
J Clin Microbiol. 2010 Jan;48(1):307-10. doi: 10.1128/JCM.01536-09. Epub 2009 Nov 11.
Although commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with GenoType Mycobacterium (Hein), 317 strains, belonging to 136 species, 61 of which had never been assayed before. INNO LiPA misidentified 20 taxa, the majority of which cross-reacted with the probes specific for Mycobacterium fortuitum and the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum group. GenoType misidentified 28 taxa, most of which cross-reacted with M. intracellulare and M. fortuitum probes; furthermore, eight species were not recognized as members of the genus Mycobacterium. Among 54 strains investigated with AccuProbe (Gen-Probe), cross-reactions were detected for nine species, with the probes aiming at the M. avium complex being most involved in cross-reactions.
尽管已经有大量商业 DNA 探针用于鉴定分枝杆菌，但对于这些探针识别不常见物种的能力却知之甚少。我们用 INNO LiPA MYCOBACTERIA（Innogenetics）和 GenoType Mycobacterium（Hein）分析了 317 株属于 136 个种的菌株，其中 61 个种以前从未进行过检测。INNO LiPA 错误鉴定了 20 个分类群，其中大多数与针对 Mycobacterium fortuitum 和 Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum 组的探针发生交叉反应。GenoType 错误鉴定了 28 个分类群，其中大多数与 M. intracellulare 和 M. fortuitum 探针发生交叉反应；此外，有 8 个种未被识别为分枝杆菌属的成员。在使用 AccuProbe（Gen-Probe）检测的 54 株菌株中，有 9 个种发生了交叉反应，与 M. avium 复合物相关的探针最容易发生交叉反应。
