Ruiz Neus, Montero Teresa, Hernandez-Borrell Jordi, Viñas Miquel
Laboratory of Microbiology, Bellvitge Biomedical Research Center, Campus de Bellvitge, University of Barcelona, Feixa Llarga s/n E-08907, L'Hospitalet, Barcelona, Spain.
Microb Drug Resist. 2003 Fall;9(3):257-64. doi: 10.1089/107662903322286463.
The outer membrane permeability of Serratia marcescens was studied by comparing porin-deficient mutants with their parental strains. Omp1-deficient strains were selected by moxalactam resistance, whereas mutants lacking the Omp2 porin were obtained by experimental infection with the SMP2 phage, whose primary receptor is the Omp2 porin. The role of porins was demonstrated in quinolone accumulation assays, where semiquantitative differences in accumulation were observed. Permeability coefficients to cephaloridine of Omp1 mutants were determined and compared with those of the parental strain. The clinical isolates S. marcescens HCPR1 and 866 showed 30- to 200-fold reduced permeability coefficients when Omp1 porin was absent.
通过比较缺乏孔蛋白的突变体与其亲本菌株，研究了粘质沙雷氏菌的外膜通透性。通过对莫西拉坦耐药性筛选出缺乏Omp1的菌株，而缺乏Omp2孔蛋白的突变体则通过用SMP2噬菌体进行实验感染获得，该噬菌体的主要受体是Omp2孔蛋白。在喹诺酮积累试验中证明了孔蛋白的作用，在该试验中观察到积累的半定量差异。测定了Omp1突变体对头孢菌素的通透性系数，并与亲本菌株进行了比较。临床分离株粘质沙雷氏菌HCPR1和866在缺乏Omp1孔蛋白时，通透性系数降低了30至200倍。
