Liguori Renato, Rommel Steffen H, Bengtsson-Palme Johan, Helmreich Brigitte, Wurzbacher Christian
Technical University of Munich, Chair of Urban Water Systems Engineering, Am Coulombwall 3, 85748 Garching, Germany.
Department of Science and Technology, Parthenope University of Naples, Centro direzionale Isola -C4, 80143, Napoli, Italy.
FEMS Microbes. 2021 Jul 22;2:xtab008. doi: 10.1093/femsmc/xtab008. eCollection 2021.
Current knowledge about the microbial communities that occur in urban road runoff is scarce. Road runoff of trafficked roads can be heavily polluted and is treated by stormwater quality improvement devices (SQIDs). However, microbes may influence the treatment process of these devices or could lead to stress resistant opportunistic microbial strains. In this study, the microbial community in the influent, effluent and the filter materials used to remove dissolved heavy metals from two different SQIDs were analyzed to determine microbial load, retention, composition, and mobile resistance genes. Although the microbes were replaced by new taxa in the effluent, there was no major retention of microbial genera. Further, the bacterial abundance of the SQIDs effluent was relatively stable over time. The heavy metal content correlated with and with microbial genera. The filter media itself was enriched with gene cassettes, carrying several heavy metal and multidrug resistance genes (e.g. and ), indicating that this is a hot spot for horizontal gene transfer. Overall, the results shed light on road runoff microbial communities, and pointed to distinct bacterial communities within the SQIDs, which subsequently influence the microbial community and the genes released with the treated water.
目前关于城市道路径流中微生物群落的知识还很匮乏。交通繁忙道路的径流可能受到严重污染，并通过雨水水质改善装置（SQIDs）进行处理。然而，微生物可能会影响这些装置的处理过程，或者可能导致产生抗逆性的机会性微生物菌株。在本研究中，分析了来自两种不同SQIDs的进水、出水以及用于去除溶解重金属的过滤材料中的微生物群落，以确定微生物负荷、截留情况、组成以及移动抗性基因。尽管出水中的微生物被新的分类群所取代，但微生物属并没有大量截留。此外，SQIDs出水的细菌丰度随时间相对稳定。重金属含量与微生物属相关。过滤介质本身富含基因盒，携带多种重金属和多药抗性基因（如 和 ），表明这是水平基因转移的热点。总体而言，研究结果揭示了道路径流微生物群落的情况，并指出了SQIDs内不同的细菌群落，这些群落随后会影响微生物群落以及处理后水中释放的基因。
