阵列描绘：一种使用DNA微阵列快速分析异常染色体的方法。
Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays.
作者信息
Fiegler H, Gribble S M, Burford D C, Carr P, Prigmore E, Porter K M, Clegg S, Crolla J A, Dennis N R, Jacobs P, Carter N P
机构信息
The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, UK.
出版信息
J Med Genet. 2003 Sep;40(9):664-70. doi: 10.1136/jmg.40.9.664.
Abstract
OBJECTIVE
The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.
METHODS
Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.
RESULTS
and
CONCLUSIONS
By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.
摘要
目的
作者描述了一种称为阵列描绘的方法，该方法可对异常染色体的内容和断点进行快速、高分辨率分析。
方法
阵列描绘在概念上与反向染色体描绘相似，涉及通过对少量流式分选染色体进行PCR产生的探针与大插入片段基因组克隆DNA微阵列的杂交。
结果
以及
结论
通过分析细胞遗传学上染色体平衡重排的患者，作者展示了阵列描绘作为一种在克隆和测序染色体重排之前定位断点的方法的有效性。
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