Akhisaroglu Mustafa, Manev Radmila, Akhisaroglu Ezgi, Uz Tolga, Manev Hari
The Psychiatric Institute, Department of Psychiatry, University of Illinois at Chicago, 1601 West Taylor Street, MC912, Chicago, IL 60612, USA.
Neuroreport. 2003 Aug 6;14(11):1471-3. doi: 10.1097/00001756-200308060-00013.
S100B is a cytokine with neurotrophic and neurite-extending activity that has been implicated in the mechanism of action of anti-depressants and in the pathobiology of aging associated disorders such as Alzheimer's disease. The antidepressant fluoxetine increases hippocampal S100B content in young adult rats. In humans, brain levels of S100B mRNA and protein increase with advancing age. We assayed hippocampal S100B protein content in young (2 month) and old (24 month) mice, and in old mice treated for 2 weeks with fluoxetine. Using quantitative Western immunoblotting and an immunoassay kit we found higher S100B content in the hippocampus of old mice. Fluoxetine treatment of old mice further increased hippocampal S100B, suggesting that aging does not interfere with fluoxetine's action on hippocampal S100B.
S100B是一种具有神经营养和神经突延伸活性的细胞因子，与抗抑郁药的作用机制以及阿尔茨海默病等与衰老相关疾病的病理生物学有关。抗抑郁药氟西汀可增加成年幼鼠海马体中的S100B含量。在人类中，S100B mRNA和蛋白质的脑水平会随着年龄的增长而增加。我们检测了年轻（2个月）和年老（24个月）小鼠以及用氟西汀治疗2周的年老小鼠海马体中的S100B蛋白质含量。使用定量蛋白质免疫印迹法和免疫分析试剂盒，我们发现年老小鼠海马体中的S100B含量更高。对年老小鼠进行氟西汀治疗可进一步增加海马体中的S100B，这表明衰老不会干扰氟西汀对海马体S100B的作用。
