海马星形胶质细胞中，细胞外S100B可刺激谷氨酸摄取。
Glutamate uptake is stimulated by extracellular S100B in hippocampal astrocytes.
作者信息
Tramontina Francine, Tramontina Ana Carolina, Souza Daniela F, Leite Marina C, Gottfried Carmem, Souza Diogo O, Wofchuk Susana T, Gonçalves Carlos-Alberto
机构信息
Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
出版信息
Cell Mol Neurobiol. 2006 Feb;26(1):81-6. doi: 10.1007/s10571-006-9099-8.
Abstract
1.S100B is a calcium-binding protein expressed and secreted by astrocytes, which has been implicated in glial-neuronal communication. Extracellular S100B appears to protect hippocampal neurons against toxic concentrations of glutamate. Here we investigated a possible autocrine role of S100B in glutamate uptake activity. 2. Astrocyte cultures were prepared of hippocampi from neonate Wistar rats. [(3)H] Glutamate uptake was measured after addition of S100B protein, antibody anti-S100B or TRTK-12, a peptide that blocks S100B activity mediated by the C-terminal region. 3.Antibody anti-S100B addition decreased glutamate uptake measured 30 min after medium replacement, without affecting cell integrity or viability. Moreover, low levels of S100B (less than 0.1 ng/mL) stimulated glutamate uptake measured immediately after medium replacement. 4. This finding reinforces the importance of astrocytes in the glutamatergic transmission, particularly the role of S100B neuroprotection against excitotoxic damage.
摘要
- S100B是一种由星形胶质细胞表达和分泌的钙结合蛋白，它与胶质-神经元通讯有关。细胞外的S100B似乎能保护海马神经元免受谷氨酸毒性浓度的影响。在此，我们研究了S100B在谷氨酸摄取活性中可能的自分泌作用。2. 从新生Wistar大鼠的海马制备星形胶质细胞培养物。在添加S100B蛋白、抗S100B抗体或TRTK-12（一种阻断由C末端区域介导的S100B活性的肽）后，测量[³H]谷氨酸摄取。3. 添加抗S100B抗体可降低更换培养基30分钟后测得的谷氨酸摄取，而不影响细胞完整性或活力。此外，低水平的S100B（低于0.1 ng/mL）在更换培养基后立即刺激谷氨酸摄取。4. 这一发现强化了星形胶质细胞在谷氨酸能传递中的重要性，特别是S100B对兴奋性毒性损伤的神经保护作用。
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