Bendjeddou D, Lalaoui K, Satta D
Department of Biology, Faculty of Sciences, Mentouri University, Constantine 25000, Algeria.
J Ethnopharmacol. 2003 Oct;88(2-3):155-60. doi: 10.1016/s0378-8741(03)00226-5.
Hot water polysaccharide extracts of Anacyclus pyrethrum (L.) Link. (family Compositae) Citrullus colocynthis (L.) Schrad. (family Cucurbitaceae) and Alpinia galanga (L.) Willd. (family Zingiberaceae) were tested for their immunostimulating activity in mice. The fractions from Anacyclus pyrethrum and Alpinia galanga showed a marked stimulating effect on the reticulo-endothelial system (RES) and increased the number of peritoneal exudate cells (PEC), and spleen cells of mice. In this case, the optimum doses were 50 and 25 mg/kg for the two fractions, respectively. On the other hand, the polysaccharide extracts of both Anacyclus pyrethrum and Alpinia galanga markedly enhanced the proliferation of the murine spleen cells in vitro using two tests (in vitro and in vivo effect). The results of the in vivo effect at a doses of 50 and 25 mg/kg, showed a stimulation index better than obtained with the in vitro effect at 50 and 25 microg/ml for Anacyclus pyrethrum and Alpinia galanga, respectively. While the extract of Citrullus colocynthis showed much weaker and variable immunostimulating activity.
对菊科植物派热菊（Anacyclus pyrethrum (L.) Link.）、葫芦科植物药西瓜（Citrullus colocynthis (L.) Schrad.）和姜科植物大高良姜（Alpinia galanga (L.) Willd.）的热水多糖提取物进行了小鼠免疫刺激活性测试。派热菊和大高良姜的提取物对小鼠的网状内皮系统（RES）有显著刺激作用，并增加了小鼠腹腔渗出细胞（PEC）和脾细胞的数量。在这种情况下，两种提取物的最佳剂量分别为50和25毫克/千克。另一方面，派热菊和大高良姜的多糖提取物通过两种试验（体外和体内效应）显著增强了小鼠脾细胞在体外的增殖。在50和25毫克/千克剂量下的体内效应结果显示，派热菊和大高良姜的刺激指数分别优于在50和25微克/毫升体外效应所获得的刺激指数。而药西瓜提取物的免疫刺激活性则弱得多且不稳定。
