Chroho Mounia, Aazza Mustapha, Bouymajane Aziz, Majdoub Yassine Oulad El, Cacciola Francesco, Mondello Luigi, Zair Touriya, Bouissane Latifa
Molecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, BP 523, Beni-Mellal 23000, Morocco.
Research Team Chemistry of Bioactive Molecules and Environment, Laboratory of Innovative Materials and Biotechnologies of Natural Resources, Faculty of Sciences, Moulay Ismail University, BP 11201, Meknes 50070, Morocco.
Plants (Basel). 2022 Dec 8;11(24):3423. doi: 10.3390/plants11243423.
In this work, polyphenols were extracted from flowers using a hydroethanolic solvent, and the obtained extract was studied for its total phenol and flavonoid contents and evaluated for its antioxidant and antibacterial capacities. The contents of total phenols and flavonoids were measured by employing gallic acid and quercetin as references, respectively, and the phenolic composition analysis was conducted using high-performance liquid chromatography combined with a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA/ESI-MS). The antioxidant capacity of the extracts was tested using a potassium ferric reducing antioxidant power (PFRAP) assay, and the antibacterial activity assay was carried out against Gram-negative bacteria ( and ) and Gram-positive bacteria ( and ) using the broth microdilution assay. The phenolic and flavonoid contents of the extracts equaled 9.53 ± 0.48 mg GAE/g dm and 1.31 ± 0.06 mg QE/g dm, respectively. The chromatographic analysis of the phenolic profile detected 26 phenolic compounds belonging to phenolic acids, flavones and flavonols, and with the caffeoylquinic acid derivatives being the major phenolic compounds present in 12 isomers. Only one organic compound, citric acid, was found. The extracts exhibited interesting antioxidant activity. Bacteriostatic activity towards and bactericidal activity against , and were determined. This study revealed that flower extracts contain phenolic compounds with interesting bioactivities.
在本研究中，使用乙醇水溶液从花朵中提取多酚，并对所得提取物的总酚和黄酮含量进行研究，同时评估其抗氧化和抗菌能力。分别以没食子酸和槲皮素为参照测定总酚和黄酮的含量，并采用高效液相色谱结合光电二极管阵列和电喷雾电离质谱（HPLC-PDA/ESI-MS）进行酚类成分分析。使用铁离子还原抗氧化能力（PFRAP）测定法测试提取物的抗氧化能力，并采用肉汤微量稀释法对革兰氏阴性菌（ 和 ）和革兰氏阳性菌（ 和 ）进行抗菌活性测定。提取物中酚类和黄酮类含量分别为9.53±0.48 mg GAE/g dm和1.31±0.06 mg QE/g dm。酚类成分的色谱分析检测到26种酚类化合物，属于酚酸、黄酮和黄酮醇类，其中咖啡酰奎尼酸衍生物是12种异构体中主要的酚类化合物。仅发现一种有机化合物，即柠檬酸。提取物表现出有趣的抗氧化活性。测定了对 的抑菌活性以及对 、 和 的杀菌活性。本研究表明， 花提取物含有具有有趣生物活性的酚类化合物。
