Braselmann H, Kulka U, Huber R, Figel H M, Zitzelsberger H
National Research Center for Environment and Health, Institute of Molecular Radiobiology, D-85764 Neuherberg, Germany.
Int J Radiat Biol. 2003 Jun;79(6):393-403. doi: 10.1080/0955300031000137805.
To investigate the DNA-proportional distribution of radiation-induced chromosome aberrations for all chromosomes of a male and a female human karyotype.
Metaphases were prepared from whole blood cultures obtained from two healthy donors and set up after irradiation with 3 Gy 220 kV X-rays. Single whole-chromosome FISH painting simultaneously with pancentromeric DNA painting were performed separately for each chromosome of the human karyotype. One thousand exclusively first-division metaphases were analysed per chromosome and donor. After statistical analysis, the data obtained were compared with theoretically expected values.
All aberration types (translocations, dicentrics) showed deviations from a DNA-proportional distribution. For both donors, chromosomes 2 and 3 exhibited significantly less and chromosome 4 more symmetrical translocations than expected. Chromosomes 15 and 22 showed more symmetrical translocations than predicted for one of the two donors. Less dicentrics than expected became apparent for chromosomes 2, 3 and 18, while more dicentrics were seen for chromosomes 15, 16 and 17. Moreover, chromosomes 4, 14 and 22 showed a significant deviation from the theoretically expected 1:1 ratio of the yields of symmetrical translocations to the yields of dicentrics.
The results from the whole-chromosome complement in two different donors confirmed published data from the analysis of single chromosomes that some human chromosomes were not involved in radiation-induced dicentrics and symmetrical translocations proportional to their DNA content. This must be taken into account if chromosome subsets for dose reconstruction are selected or if whole genomic frequencies have to be calculated from partial genome analysis.
研究男性和女性人类核型中所有染色体辐射诱导染色体畸变的DNA比例分布。
从两名健康供体的全血培养物中制备中期相，并在3 Gy 220 kV X射线照射后进行设置。对人类核型的每条染色体分别进行单条全染色体荧光原位杂交（FISH）染色同时进行着丝粒全DNA染色。每个染色体和供体分析1000个仅处于第一次分裂的中期相。经过统计分析后，将获得的数据与理论预期值进行比较。
所有畸变类型（易位、双着丝粒染色体）均显示出与DNA比例分布的偏差。对于两名供体，2号和3号染色体的对称易位明显少于预期，而4号染色体的对称易位则更多。15号和22号染色体在两名供体中的一名中显示出比预测更多的对称易位。2号、3号和18号染色体的双着丝粒染色体比预期少，而15号、16号和17号染色体的双着丝粒染色体则更多。此外，4号、14号和22号染色体的对称易位产量与双着丝粒染色体产量的理论预期1:1比例存在显著偏差。
来自两名不同供体的全染色体组的结果证实了单条染色体分析的已发表数据，即一些人类染色体在辐射诱导的双着丝粒染色体和对称易位中与其DNA含量不成比例。如果选择用于剂量重建的染色体亚组或必须从部分基因组分析计算全基因组频率时，必须考虑这一点。
