Virsik-Peuckert P, Rave-Fränk M, Langebrake U, Schmidberger H
Institut für Medizinische Physik und Biophysik, Universität Göttingen, Germany.
Radiat Res. 1997 Sep;148(3):209-15.
Plateau-phase human dermal fibroblasts and whole blood G0 lymphocytes obtained from the same three healthy donors were irradiated with different doses of 200 kV X rays. The genomic yields of dicentrics were evaluated in the first postirradiation mitosis. Fluorescence in situ hybridization was used to "paint" the chosen whole chromosomes. The yields of reciprocal translocations were scored for all donors for chromosome 4, and also for the third donor for chromosomes 7 and 9. The amounts of reciprocal translocations were scored in the first as well as in later (4th or 5th) postirradiation cell divisions. The yields of dicentrics involving the painted chromosomes were scored for one donor as well. The yields of dicentrics observed in the lymphocytes and in the skin fibroblasts from the same individual were significantly different. Yields of dicentrics were much lower in fibroblasts than in lymphocytes. This was observed for all doses up to 4 Gy for all three donors studied. Fibroblasts from two individuals were also irradiated with 6 Gy. The interindividual variability was similar in both cell types. The yields of reciprocal translocations in lymphocytes and in fibroblasts were similar. The yields of reciprocal translocations were practically constant when comparing the first mitotic divisions and later divisions. The observed ratio of translocations:dicentrics was higher in fibroblasts than in lymphocytes, where it was about 1 for one donor and about 2 for the other two donors. Significant differences between the yields of dicentrics and translocations as well as between the shapes of their dose responses were observed in lymphocytes and fibroblasts from the same individual. Our experimental data suggest that repair processes with different efficiencies or different repair processes might be active in cells of different types of tissue.
从三名健康供体获取的处于平台期的人皮肤成纤维细胞和全血G0淋巴细胞，用不同剂量的200 kV X射线进行照射。在照射后的第一次有丝分裂中评估双着丝粒的基因组产额。使用荧光原位杂交技术对选定的整条染色体进行“描绘”。对所有供体的4号染色体以及第三名供体的7号和9号染色体的相互易位产额进行评分。在照射后的第一次以及随后（第4次或第5次）细胞分裂中对相互易位的数量进行评分。还对一名供体涉及描绘染色体的双着丝粒产额进行了评分。在同一个体的淋巴细胞和皮肤成纤维细胞中观察到的双着丝粒产额存在显著差异。成纤维细胞中的双着丝粒产额远低于淋巴细胞。在研究的所有三名供体中，直至4 Gy的所有剂量下均观察到这种情况。来自两名个体的成纤维细胞也用6 Gy进行了照射。两种细胞类型的个体间变异性相似。淋巴细胞和成纤维细胞中的相互易位产额相似。比较第一次有丝分裂和后续分裂时，相互易位产额实际上是恒定的。在成纤维细胞中观察到的易位与双着丝粒的比例高于淋巴细胞，在淋巴细胞中，一名供体的该比例约为1，另外两名供体的约为2。在同一个体的淋巴细胞和成纤维细胞中，观察到双着丝粒和易位产额之间以及它们的剂量反应形状之间存在显著差异。我们的实验数据表明，不同效率的修复过程或不同的修复过程可能在不同类型组织的细胞中活跃。
