Mosbech H, Nielsen N H, Dirksen A, Launbjerg J, Biering I, Søborg M
Medical Department TTA, National University Hospital, Rigshospitalet, Copenhagen, Denmark.
Allergol Immunopathol (Madr). 1992 Nov-Dec;20(6):220-4.
Two hundred adult patients referred to a regional outpatient allergy clinic were skin prick tested and their specific IgE against ten inhalant allergens was measured by three different assays: the classical radioallergosorbent assay, Phadebas RAST (P-RAST), (Pharmacia AB, Uppsala, Sweden), the multi-allergosorbent chemiluminescent assay CLA (MAST), (Dome/Hollister-Stier, Miles Ltd., UK), and the immunocheminometric assay, MAGIC LITE SQ Specific IgE (ML), (ALK Laboratorierne, Hørsholm, Denmark). Skin prick test (SPT) was performed with extracts from the same companies. Marked differences between the assays were seen especially in the low range. Specific IgE was detected in 17% of the samples by P-RAST, in 66% by CLA, and in 17% by ML. Frequency distribution of P-RAST and ML measurements showed a peak at zero and a gradual decline towards larger values, whereas distribution for CLA was U-shaped with a peak at zero and another peak close to maximum reactions. Kendall's rank correlation coefficient being 0.80 for P-RAST vs. CLA. Correlation coefficient between measurements of specific IgE and SPT was high for CLA (0.65). Even in the low-level subgroup where IgE was detected by CLA only, a high correlation (0.60) between CLA and SPT was observed. In conclusion marked differences were observed between the three assays for specific IgE measurement. The CLA system seems to be more sensitive than the conventional P-RAST and ML, but further studies should reveal whether this extra sensitivity is clinically useful.
200名转诊至某地区门诊过敏诊所的成年患者接受了皮肤点刺试验，并通过三种不同的检测方法测量了他们针对十种吸入性变应原的特异性IgE：经典的放射变应原吸附试验、Phadebas RAST（P-RAST）（瑞典乌普萨拉法玛西亚公司）、多变应原吸附化学发光试验CLA（MAST）（英国迈尔斯有限公司陶氏/霍利斯特-斯蒂尔分公司）以及免疫化学发光分析法MAGIC LITE SQ特异性IgE（ML）（丹麦霍斯霍尔姆ALK实验室）。皮肤点刺试验（SPT）使用的是来自相同公司的提取物。尤其在低水平范围内，各检测方法之间存在显著差异。P-RAST在17%的样本中检测到特异性IgE，CLA为66%，ML为17%。P-RAST和ML测量值的频率分布在零处有一个峰值，并随着数值增大逐渐下降，而CLA的分布呈U形，在零处有一个峰值，另一个峰值接近最大反应值。P-RAST与CLA的肯德尔等级相关系数为0.80。CLA检测特异性IgE与SPT之间的相关系数较高（0.65）。即使在仅通过CLA检测到IgE的低水平亚组中，也观察到CLA与SPT之间有较高的相关性（0.60）。总之，三种特异性IgE检测方法之间存在显著差异。CLA系统似乎比传统的P-RAST和ML更敏感，但进一步的研究应揭示这种额外的敏感性在临床上是否有用。
