Onoue Y, Murakami G, Takano M, Kayahara M, Adachi Y, Matsuno M, Adachi Y, Okada T
Department of Pediatrics, Toyama Medical and Pharmaceutical University.
Arerugi. 1993 Jul;42(7):846-53.
The new IgE specific antibody assay kit (Magic Lite SQ) was evaluated by comparing its results with those obtained by the Phadebas RAST and the skin prick tests. Sera from allergic children were used in the tests. The correlation coefficients between the Magic Lite SQ and the Phadebas RAST were as follows: 0.87 for D.p., 0.89 for D.f., 0.87 for HD1, 0.55 for HD2, 0.76 for orchard grass, 0.93 for cat dander, 0.78 for Alternaria, 0.88 for egg white, 0.75 for egg york, 0.81 for milk, 0.75 for say bean, and 0.60 for shrimp. The concordance rate of the two methods was high, except on the case of HD2. The sensitivity and specificity of Magic Lite SQ to the skin prick test was high, except on the case of HD2 and orchard grass. Dilution tests using samples from D.f. positive patients showed a good linearity and excellent quantitativeness. When mite allergen was added to D.f. positive pool serum, mite specific IgE antibody yielded an excellent inhibition curve of up to a maximum of 96%. Since the Magic Lite SQ requires only a small sample of serum and can completed the test in a short time, it is a useful and specific IgE antibody assay kit.
通过将新型IgE特异性抗体检测试剂盒（Magic Lite SQ）的检测结果与Phadebas RAST和皮肤点刺试验的结果进行比较，对其进行了评估。检测中使用了过敏儿童的血清。Magic Lite SQ与Phadebas RAST之间的相关系数如下：粉尘螨为0.87，屋尘螨为0.89，混合组1为0.87，混合组2为0.55，果园草为0.76，猫皮屑为0.93，链格孢属为0.78，蛋清为0.88，蛋黄为0.75，牛奶为0.81，大豆为0.75，虾为0.60。除混合组2外，两种方法的一致性率较高。Magic Lite SQ对皮肤点刺试验的敏感性和特异性较高，除混合组2和果园草外。使用屋尘螨阳性患者的样本进行的稀释试验显示出良好的线性和出色的定量性。当将螨过敏原添加到屋尘螨阳性混合血清中时，螨特异性IgE抗体产生了高达96%的出色抑制曲线。由于Magic Lite SQ仅需要少量血清样本，并且可以在短时间内完成检测，因此它是一种有用且特异的IgE抗体检测试剂盒。
