Zvereva Elena, Serebrov Valery, Glupov Viktor, Dubovskiy Ivan
Section of Ecology, Department of Biology, University of Turku, Turku FIN-20014, Finland.
Comp Biochem Physiol C Toxicol Pharmacol. 2003 Aug;135(4):383-91. doi: 10.1016/s1532-0456(03)00115-7.
We compared the general activity and heavy metal resistance of non-specific esterases in two populations of the leaf beetle Chrysomela lapponica from habitats severely contaminated by heavy metals (mostly Ni and Cu) and two populations from unpolluted habitats. Concentrations of Ni and Cu in adult beetles from the most polluted site were 7.7 and 3.6 times higher that in beetles from unpolluted habitats. Larval esterases showed higher activity and lower susceptibility to heavy metals than esterases of adults. Larval esterase activity did not differ between populations from polluted and unpolluted sites, but adult beetles from polluted localities had lower esterase activity than beetles from unpolluted habitats. Both Cu and Ni sulfates in millimolar concentrations in vitro suppressed esterase activity of larvae from unpolluted habitats, but caused no negative effect on esterases of larvae from polluted sites. Similarly, inhibition of adult esterase activity by Ni was stronger in beetles from unpolluted localities than in beetles from polluted localities. This indicates that resistance of non-specific esterases to heavy metals is higher in leaf beetle populations from contaminated environment.
我们比较了来自重金属（主要是镍和铜）严重污染栖息地的两种叶甲（Chrysomela lapponica）种群以及来自未受污染栖息地的两种叶甲种群中非特异性酯酶的一般活性和重金属抗性。来自污染最严重地点的成年甲虫体内镍和铜的浓度分别比来自未受污染栖息地的甲虫高出7.7倍和3.6倍。幼虫酯酶比成虫酯酶表现出更高的活性和更低的重金属敏感性。来自污染和未污染地点的幼虫酯酶活性没有差异，但来自污染地区的成年甲虫酯酶活性低于来自未受污染栖息地的甲虫。体外毫摩尔浓度的硫酸铜和硫酸镍均抑制了来自未受污染栖息地幼虫的酯酶活性，但对来自污染地点幼虫的酯酶没有负面影响。同样，镍对未受污染地区甲虫成虫酯酶活性的抑制作用比对污染地区甲虫的抑制作用更强。这表明来自受污染环境的叶甲种群中非特异性酯酶对重金属的抗性更高。
