Lee Eun-Jin, Kim Keun-Young, Gu Tae-Hyung, Moon Jung-Il, Kim In-Beom, Lee Mun-Yong, Oh Su-Ja, Chun Myung-Hoon
Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, South Korea.
Brain Res. 2003 Oct 3;986(1-2):174-80. doi: 10.1016/s0006-8993(03)03250-5.
This study investigated the expression and cellular localization of neuronal nitric oxide synthase in the rat retina following optic nerve transection (ONT). In the normal rat retina, nNOS immunoreactivity was localized to amacrine cells and displaced amacrine cells. A few bipolar cells were also labeled. In the axotomized retina, ganglion cells showed nNOS immunoreactivity from 3 days after ONT, and these cells increased in number, peaking 5 days after ONT. Quantitative evaluation using immunoblotting confirmed that nNOS expression showed a peak value (255% of control levels) 5 days after ONT and decreased to 137% of controls by 28 days. These findings suggest that axotomized ganglion cells degenerate via NO-mediated excitotoxicity.
本研究调查了视神经横断（ONT）后大鼠视网膜中神经元型一氧化氮合酶的表达及细胞定位。在正常大鼠视网膜中，nNOS免疫反应性定位于无长突细胞和移位无长突细胞。少数双极细胞也有标记。在轴突切断的视网膜中，从ONT后3天起，神经节细胞显示nNOS免疫反应性，且这些细胞数量增加，在ONT后5天达到峰值。使用免疫印迹法进行的定量评估证实，nNOS表达在ONT后5天出现峰值（为对照水平的255%），到28天时降至对照水平的137%。这些发现表明，轴突切断的神经节细胞通过NO介导的兴奋性毒性发生退化。
