Oshitani Nobuhide, Hato Fumihiko, Kitagawa Seiichi, Watanabe Kenji, Fujiwara Yasuhiro, Higuchi Kazuhide, Matsumoto Takayuki, Arakawa Tetsuo
Department of Gastroenterology, Osaka City University Graduate School of Medicine, Osaka 545-8585 Japan.
Clin Diagn Lab Immunol. 2003 Sep;10(5):856-61. doi: 10.1128/cdli.10.5.856-861.2003.
Dysregulation of immune responses to intestinal exogenous antigens contributes to the pathogenesis of inflammatory bowel disease, but the specific antigen responsible for the pathogenesis of inflammatory bowel disease is unknown. We measured serum antibody titers against Caenorhabditis elegans antigens. Immunoglobulin G (IgG) and IgG subclass anti-C. elegans antibodies in serum samples from 29 patients with ulcerative colitis, 30 patients with Crohn's disease, 7 patients with intestinal Behçet's disease, and 11 healthy controls were measured by enzyme-linked immunosorbent assay. Serum IgG and IgG2 antibody titers against C. elegans were significantly higher in patients with inflammatory bowel disease than in controls. Antibody levels were not affected by age, gender, disease activity, extent of disease, or small bowel involvement. The anti-C. elegans antibody titer was significantly lower in patients with Crohn's disease taking mesalazine or sulfasalazine than in patients not taking these drugs. The increased immune responses to C. elegans found in patients with inflammatory bowel disease reflect dysregulated immune responses to enteric antigens, which might play a role in the pathogenesis of inflammatory bowel disease.
肠道对外源抗原免疫反应的失调促成了炎症性肠病的发病机制，但导致炎症性肠病发病的具体抗原尚不清楚。我们检测了血清中针对秀丽隐杆线虫抗原的抗体滴度。通过酶联免疫吸附测定法检测了29例溃疡性结肠炎患者、30例克罗恩病患者、7例肠道白塞病患者和11名健康对照者血清样本中的免疫球蛋白G（IgG）及IgG亚类抗秀丽隐杆线虫抗体。炎症性肠病患者血清中针对秀丽隐杆线虫的IgG和IgG2抗体滴度显著高于对照组。抗体水平不受年龄、性别、疾病活动度、疾病范围或小肠受累情况的影响。服用美沙拉嗪或柳氮磺胺吡啶的克罗恩病患者的抗秀丽隐杆线虫抗体滴度显著低于未服用这些药物的患者。炎症性肠病患者中发现的对秀丽隐杆线虫免疫反应增强反映了对肠道抗原的免疫反应失调，这可能在炎症性肠病的发病机制中起作用。
