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DNA与DNA结合蛋白的相互作用。大肠杆菌蛋白HD及其核酸复合物的特性研究。

Interaction of DNA with DNA-binding proteins. The characterization of protein HD from Escherichia coli and its nucleic acid complexes.

作者信息

Berthold V, Geider K

出版信息

Eur J Biochem. 1976 Dec 11;71(2):443-9. doi: 10.1111/j.1432-1033.1976.tb11132.x.

DOI:10.1111/j.1432-1033.1976.tb11132.x
PMID:12966
Abstract

DNA-binding protein HD with a monomer molecular weight of 9000 was isolated from Escherichia coli cells. The protein occurs as a tetramer under native conditions and binds to single and double-stranded DNA and also to RNA. DNA complexed with protein HD is a poor template for DNA synthesis by E. coli polymerase I, II or III holoenzyme. Exonuclease III is hindered in degrading HD-protein-covered double-stranded DNA, whereas exonuclease I can digest complexed single-stranded DNA. Transcription is liqhtly stimulated in the presence of protein HD.

摘要

从大肠杆菌细胞中分离出了单体分子量为9000的DNA结合蛋白HD。该蛋白在天然条件下以四聚体形式存在，可与单链和双链DNA以及RNA结合。与蛋白HD复合的DNA是大肠杆菌聚合酶I、II或III全酶进行DNA合成的不良模板。核酸外切酶III在降解被HD蛋白覆盖的双链DNA时受到阻碍，而核酸外切酶I可以消化复合的单链DNA。在蛋白HD存在的情况下，转录受到轻微刺激。

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