Zentgraf H, Berthold V, Geider K
Biochim Biophys Acta. 1977 Feb 16;474(4):629-38. doi: 10.1016/0005-2787(77)90082-x.
Three DNA binding proteins from Escherichia coli cells have been complexed with single-stranded phage fd DNA. Electron microscopy reveals granular substructures in the complexes formed with protein HD. In complexes of DNA unwinding protein with fd DNA both protein HD and phage-coded gene 5 protein partially displace the unwinding protein which results in the formation of structures characteristic for the DNA complexes formed with either protein HD or gene 5 protein alone. Combination of protein HD with double-stranded phage T7 DNA leads to a progressive folding and condensing of the genome. The structures observed are discussed in relation to current concepts of the packing of DNA in protein complexes.
来自大肠杆菌细胞的三种DNA结合蛋白已与单链噬菌体fd DNA形成复合物。电子显微镜显示，与蛋白质HD形成的复合物中有颗粒状亚结构。在解旋蛋白与fd DNA的复合物中，蛋白质HD和噬菌体编码的基因5蛋白都会部分取代解旋蛋白，从而导致形成与单独与蛋白质HD或基因5蛋白形成的DNA复合物特有的结构。蛋白质HD与双链噬菌体T7 DNA结合会导致基因组逐渐折叠和浓缩。结合当前关于DNA在蛋白质复合物中包装的概念，对观察到的结构进行了讨论。
