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DNA与DNA结合蛋白的相互作用。III. 蛋白质复合噬菌体fd DNA在大肠杆菌原生质体中的感染性。

Interaction of DNA with DNA binding proteins. III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts.

作者信息

Uhlmann A, Geider K

出版信息

Biochim Biophys Acta. 1977 Feb 16;474(4):639-45. doi: 10.1016/0005-2787(77)90083-1.

DOI:10.1016/0005-2787(77)90083-1
PMID:319836
Abstract

Complex formation of circular, single-stranded phage fd DNA with Escherichia coli DNA binding protein HD or phage fd gene 5 protein keeps infection of E. coli spheroplasts at the level of free phage DNA, whereas complexes of this DNA with E. coli DNA unwinding protein show a strongly reduced efficiency of transfection. Displacement of the unwinding protein by HD protein or gene 5 protein also maintains the poor adsorption of the complexes to spheroplasts. Free E. coli DNA unwinding protein and residual amounts of this protein bound to the DNA may interfere with the adsorption and the uptake of the phage genome.

摘要

环状单链噬菌体fd DNA与大肠杆菌DNA结合蛋白HD或噬菌体fd基因5蛋白形成的复合物，使大肠杆菌原生质球的感染维持在游离噬菌体DNA的水平，而该DNA与大肠杆菌DNA解旋蛋白形成的复合物转染效率则大幅降低。HD蛋白或基因5蛋白取代解旋蛋白也会使复合物对原生质球的吸附能力较弱。游离的大肠杆菌DNA解旋蛋白以及与DNA结合的该蛋白残留量可能会干扰噬菌体基因组的吸附和摄取。

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Interaction of DNA with DNA binding proteins. III. Infectivity of protein-complexed phage fd DNA in Escherichia coli spheroplasts.DNA与DNA结合蛋白的相互作用。III. 蛋白质复合噬菌体fd DNA在大肠杆菌原生质体中的感染性。
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