Collett Helen, Butowt Rafal, Smith James, Farrant Jill, Illing Nicola
Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa.
J Exp Bot. 2003 Nov;54(392):2593-5. doi: 10.1093/jxb/erg285. Epub 2003 Sep 9.
One of the desiccation-tolerant mechanisms of the resurrection plant, Xerophyta humilis, is the ability to shut down photosynthesis reversibly. The X. humilis psbR and ChlP genes, encoding the 10 kDa polypeptide of photosystem II (PSII) and a geranylgeranyl reductase, respectively, were isolated in a differential display screen as dehydration-down-regulated and rehydration-up-regulated transcripts. Two other PSII genes, psbA (chloroplast-encoded) and psbP (nuclear-encoded), isolated by degenerate primer PCR, display a similar trend in expression.
复苏植物旱生草(Xerophyta humilis)的一种耐干燥机制是能够可逆地关闭光合作用。分别编码光系统II(PSII)的10 kDa多肽和香叶基香叶基还原酶的旱生草psbR和ChlP基因,在差异显示筛选中被分离为脱水下调和复水上调转录本。通过简并引物PCR分离的另外两个PSII基因psbA(叶绿体编码)和psbP(核编码)在表达上呈现出类似的趋势。