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在复苏植物旱生萱草的脱水-复水循环过程中,光合基因的转录存在差异。

Photosynthetic genes are differentially transcribed during the dehydration-rehydration cycle in the resurrection plant, Xerophyta humilis.

作者信息

Collett Helen, Butowt Rafal, Smith James, Farrant Jill, Illing Nicola

机构信息

Department of Molecular and Cell Biology, University of Cape Town, Rondebosch 7701, South Africa.

出版信息

J Exp Bot. 2003 Nov;54(392):2593-5. doi: 10.1093/jxb/erg285. Epub 2003 Sep 9.

Abstract

One of the desiccation-tolerant mechanisms of the resurrection plant, Xerophyta humilis, is the ability to shut down photosynthesis reversibly. The X. humilis psbR and ChlP genes, encoding the 10 kDa polypeptide of photosystem II (PSII) and a geranylgeranyl reductase, respectively, were isolated in a differential display screen as dehydration-down-regulated and rehydration-up-regulated transcripts. Two other PSII genes, psbA (chloroplast-encoded) and psbP (nuclear-encoded), isolated by degenerate primer PCR, display a similar trend in expression.

摘要

复苏植物旱生草(Xerophyta humilis)的一种耐干燥机制是能够可逆地关闭光合作用。分别编码光系统II(PSII)的10 kDa多肽和香叶基香叶基还原酶的旱生草psbR和ChlP基因,在差异显示筛选中被分离为脱水下调和复水上调转录本。通过简并引物PCR分离的另外两个PSII基因psbA(叶绿体编码)和psbP(核编码)在表达上呈现出类似的趋势。

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