Vidović Marija, Battisti Ilaria, Pantelić Ana, Morina Filis, Arrigoni Giorgio, Masi Antonio, Jovanović Sonja Veljović
Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia.
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
Plants (Basel). 2022 Apr 28;11(9):1199. doi: 10.3390/plants11091199.
The resurrection plant Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of HO scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived HO/HO activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement.
复苏植物 Panc. 能在长时间干旱期存活，并在浇水后一天内完全恢复代谢功能。本研究旨在鉴定参与耐旱性的关键候选基因和途径。我们结合了差异转录组学和蛋白质组学、酚类和糖类分析、细胞壁聚合物的傅里叶变换红外光谱分析以及光合电子传递（PET）链的详细分析。蛋白质组学分析允许对1192个不同的蛋白质组进行相对定量，其中408个在水合叶片（HL）和干燥叶片（DL）之间差异丰富。几乎所有与光合过程相关的差异丰富蛋白质含量都较低，而叶绿素荧光测量表明从线性PET向循环电子传递（CET）转变。DL中HO清除酶、抗坏血酸-谷胱甘肽循环成分、过氧化氢酶、过氧化物酶、铁和锰超氧化物歧化酶（SOD）的水平降低。然而，六种类萌发素蛋白（GLP）、四种铜/锌SOD同工型、三种多酚氧化酶和22种晚期胚胎发生丰富蛋白（LEAPs；主要是LEA4和脱水素）是干旱诱导型的。干旱引发了与GLP衍生的HO/HO活性和果胶去甲基化相关的细胞壁重塑。这项全面的研究有助于理解干旱期间主要代谢途径的作用和调控，旨在提高作物的耐旱性。
