Gabier Hawwa, Tabb David L, Farrant Jill M, Rafudeen Mohamed Suhail
Department of Molecular and Cell Biology, University of Cape Town, Private Bag X3, Rondebosch 7701, South Africa.
Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7700, South Africa.
Life (Basel). 2021 Nov 16;11(11):1242. doi: 10.3390/life11111242.
Vegetative desiccation tolerance, or the ability to survive the loss of ~95% relative water content (RWC), is rare in angiosperms, with these being commonly called resurrection plants. It is a complex multigenic and multi-factorial trait, with its understanding requiring a comprehensive systems biology approach. The aim of the current study was to conduct a label-free proteomic analysis of leaves of the resurrection plant in response to desiccation. A targeted metabolomics approach was validated and correlated to the proteomics, contributing the missing link in studies on this species. Three physiological stages were identified: an early response to drying, during which the leaf tissues declined from full turgor to a RWC of ~80-70%, a mid-response in which the RWC declined to 40% and a late response where the tissues declined to 10% RWC. We identified 517 distinct proteins that were differentially expressed, of which 253 proteins were upregulated and 264 were downregulated in response to the three drying stages. Metabolomics analyses, which included monitoring the levels of a selection of phytohormones, amino acids, sugars, sugar alcohols, fatty acids and organic acids in response to dehydration, correlated with some of the proteomic differences, giving insight into the biological processes apparently involved in desiccation tolerance in this species.
营养体干燥耐受性，即能够在相对含水量（RWC）损失约95%的情况下存活，在被子植物中较为罕见，这类植物通常被称为复苏植物。这是一个复杂的多基因和多因素性状，对其理解需要采用全面的系统生物学方法。本研究的目的是对复苏植物叶片进行无标记蛋白质组学分析，以研究其对干燥的响应。一种靶向代谢组学方法得到验证，并与蛋白质组学相关联，填补了该物种研究中的缺失环节。确定了三个生理阶段：干燥早期响应阶段，在此期间叶片组织从完全膨压状态下降到相对含水量约80 - 70%；中期响应阶段，相对含水量下降到40%；后期响应阶段，组织相对含水量下降到10%。我们鉴定出517种差异表达的独特蛋白质，其中253种蛋白质上调，264种蛋白质下调，以响应三个干燥阶段。代谢组学分析包括监测一系列植物激素、氨基酸、糖类、糖醇、脂肪酸和有机酸在脱水过程中的水平，与一些蛋白质组差异相关，从而深入了解该物种中显然与干燥耐受性有关的生物学过程。
