Feng Xiao, Yang Zhao, Wang Xiurong
College of Forestry, Guizhou University, Guiyang, Huaxi, China.
Institute for Forest Resources & Environment of Guizhou, Guizhou University, Guiyang, Huaxi, China.
PeerJ. 2021 Apr 1;9:e10933. doi: 10.7717/peerj.10933. eCollection 2021.
has broad economic benefits and excellent drought resistance; however, its drought response, adaptation, and recovery processes remain unclear.
In this study, the response, tolerance, and recovery processes of leaves and roots under drought stress were determined by Illumina sequencing.
Under drought stress, reduced its light-capturing ability and composition of its photosynthetic apparatus, thereby reducing photosynthesis to prevent photo-induced chloroplast reactive oxygen damage during dehydration. The phenylpropanoid biosynthesis process in the roots was suppressed, , , and other genes, which may play important roles in protecting the cell membrane's permeability in root tissues. During the rehydration phase, fatty acid biosynthesis in roots was repressed. Weighted correlation network analysis (WGCNA) screened modules that were positively or negatively correlated with physiological traits. The real-time quantitative PCR (RT-qPCR) results indicated the reliability of the transcriptomic data.
These findings provide valuable information for identifying important components in the drought signaling network and enhances our understanding of the molecular mechanisms by which responds to drought stress.
具有广泛的经济效益和出色的抗旱性；然而，其干旱响应、适应和恢复过程仍不清楚。
在本研究中，通过Illumina测序确定了干旱胁迫下叶片和根系的响应、耐受性和恢复过程。
在干旱胁迫下，降低了其光捕获能力和光合机构的组成，从而降低光合作用以防止脱水过程中光诱导的叶绿体活性氧损伤。根系中的苯丙烷生物合成过程受到抑制， 、 、 等基因，可能在保护根系组织中细胞膜的通透性方面发挥重要作用。在复水阶段，根系中的脂肪酸生物合成受到抑制。加权相关网络分析（WGCNA）筛选出与生理性状呈正相关或负相关的模块。实时定量PCR（RT-qPCR）结果表明转录组数据的可靠性。
这些发现为识别干旱信号网络中的重要成分提供了有价值的信息，并增强了我们对 对干旱胁迫响应的分子机制的理解。
