Parra Dolores, González Alvaro, García-Villarreal Luis, Martínez J Alfredo
Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
Alcohol Clin Exp Res. 2003 Aug;27(8):1293-8. doi: 10.1097/01.ALC.0000081623.25175.7E.
The 2-keto[1-13C]isocaproate oxidation measurement has been shown as a helpful tool in the in vivo assessment of liver mitochondrial function.
The aim of this work was to study the variability of the 2-keto[1-13C]isocaproate breath test in 24 healthy controls (8 men and 16 women) and to evaluate its clinical usefulness in 20 patients (14 men and 6 women) with liver disease (7 men with history of alcoholism). Breath test was performed by measuring 13CO2 enrichment in breath before and after the oral administration of the tracer and by using isotope ratio mass spectrometry.
The intrasubject and intersubject variability of the percentage of tracer oxidized were 8 and 14%, respectively. The 2-keto[1-13C]isocaproate oxidation in women was faster (p = 0.004) and tended to be higher (p = 0.050) than in men. The percentage of oxidized tracer was lower in those patients with alcoholic liver disease than in healthy volunteers (p = 0.001) and in nonalcoholic patients (p = 0.003).
The percentage of tracer oxidized appears as a convenient parameter to detect impairment in liver mitochondrial oxidation related to alcoholism by the 2-keto[1-13C]isocaproate breath test, establishing different cutoff values depending on gender.
2-酮-[1-¹³C]异己酸氧化测量已被证明是体内评估肝线粒体功能的有用工具。
本研究旨在探讨24名健康对照者（8名男性和16名女性）的2-酮-[1-¹³C]异己酸呼气试验的变异性，并评估其在20例肝病患者（14名男性和6名女性，其中7名男性有酗酒史）中的临床应用价值。呼气试验通过测量口服示踪剂前后呼出气体中¹³CO₂的富集情况，并使用同位素比率质谱法进行。
示踪剂氧化百分比的受试者内和受试者间变异性分别为8%和14%。女性的2-酮-[1-¹³C]异己酸氧化速度更快（p = 0.004），且氧化百分比有高于男性的趋势（p = 0.050）。酒精性肝病患者的示踪剂氧化百分比低于健康志愿者（p = 0.001）和非酒精性肝病患者（p = 0.003）。
通过2-酮-[1-¹³C]异己酸呼气试验，示踪剂氧化百分比似乎是检测与酗酒相关的肝线粒体氧化损伤的一个方便参数，可根据性别确定不同的临界值。
