Trumel C, Verwaerde P, Rascol A, Braun J P
Department of Companion Animal Medicine, National Veterinary School, Toulouse, France.
Vet Clin Pathol. 2003;32(3):140-2. doi: 10.1111/j.1939-165x.2003.tb00327.x.
The Reflovet system is designed for chemical analysis of whole blood. However, plasma or serum is recommended for potassium analysis because of possible interference from RBC potassium. Because RBC potassium concentration is low in most canine erythrocytes, however, there should be little or no interference.
The objective of this study was to compare potassium results obtained in whole blood and in plasma from dogs using the Reflovet system.
Blood samples were collected from 104 dogs into lithium-heparin tubes. The potassium concentration was measured in whole blood, and subsequently the PCV was measured. Samples were centrifuged and the potassium concentration was measured in plasma. Comparisons were made using Deming's regression and Bland-Altman difference plots.
There was very good correlation between results of potassium measurements in whole blood and plasma (r = 0.93). Potassium values were moderately lower in whole blood: Potassium(blood) = 0.912 x Potassium(plasma)+ 0.119. Hemolysis had a negligible effect on the results, but the difference increased with the PCV value. In more than 90% of samples, the difference between the 2 measurements was <or= 0.3 mmol/L.
There is only a negligible difference in most cases between potassium values in canine plasma and whole blood using the Reflovet system.
Reflovet系统专为全血化学分析而设计。然而，由于红细胞钾可能产生干扰，建议使用血浆或血清进行钾分析。不过，由于大多数犬类红细胞中的红细胞钾浓度较低，因此干扰应很小或不存在。
本研究的目的是比较使用Reflovet系统在犬全血和血浆中获得的钾结果。
从104只犬采集血样至锂肝素管中。测量全血中的钾浓度，随后测量血细胞比容。将样本离心并测量血浆中的钾浓度。使用Deming回归和Bland-Altman差异图进行比较。
全血和血浆中钾测量结果之间存在非常好的相关性（r = 0.93）。全血中的钾值略低：钾（血液）= 0.912×钾（血浆）+ 0.119。溶血对结果的影响可忽略不计，但差异随血细胞比容值增加。在超过90%的样本中，两次测量之间的差异≤0.3 mmol/L。
使用Reflovet系统时，犬血浆和全血中的钾值在大多数情况下差异可忽略不计。
