Shim Young-Hun, Choi Jung-Ho, Park Chan-Dong, Lim Chul-Joo, Cho Jung-Hee, Kim Hong-Jin
College of Pharmacy, Chung Ang University, 221, Huksuk-Dong, Dongjak-Ku, Seoul 156-756, Korea.
Arch Pharm Res. 2003 Aug;26(8):601-5. doi: 10.1007/BF02976708.
Traditional taxonomic methods used for the identification and differentiation of ginsengs rely primarily on morphological observations or physiochemical methods, which cannot be used efficiently when only powdered forms or shredded material is available. Randomly amplified polymorphic DNA (RAPD) was used to determine the unique DNA profiles that are characteristic not only of the genus Panax but also of various Panax subgroups collected from five different countries. RAPD results of OP-5A primer showed a specific single band that is characteristic of all ginseng samples. RAPD results of OP-13B primer demonstrated that OP-13B primer could be used as a unique RAPD marker to differentiate Panax species. These results support that this approach could be applied to distinguish Korean Ginseng (Panax ginseng) from others at the molecular level.
用于人参鉴定和区分的传统分类方法主要依赖形态学观察或理化方法，当只有粉末状或切碎的材料时，这些方法无法有效使用。随机扩增多态性DNA（RAPD）被用于确定独特的DNA图谱，这些图谱不仅是人参属的特征，也是从五个不同国家收集的各种人参亚组的特征。OP-5A引物的RAPD结果显示出一条特定的单带，这是所有人参样品的特征。OP-13B引物的RAPD结果表明，OP-13B引物可作为区分人参种类的独特RAPD标记。这些结果支持这种方法可用于在分子水平上区分高丽参（Panax ginseng）与其他品种。
