Buzaleh A M, Enriquez de Salamanca R, del Carmen Batlle A M
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET-FCEN, UBA, Ciudad Universitaria, Pabellón II, Argentine.
J Pharmacol Toxicol Methods. 1992 Dec;28(4):191-7. doi: 10.1016/1056-8719(92)90003-j.
The effects of isoflurane, a commonly used volatile anesthetic, on the activity of some haem enzymes in liver, kidney, and blood, and glucose content in liver and blood were studied. Mice were injected with different doses of the drug (0.5-6 mL/kg) and killed at varying intervals after injection (5-240 min). Within this dose range, optimal effects on alteration of haem metabolism were obtained at 2 mL/kg. The time-response profile for each enzyme was different. Blood porphobilinogenase (PBGase) and deaminase showed lower activities 20 min after anesthesia. This diminution coupled with the induction of delta-aminolevulinate synthetase activity observed soon after anesthesia (5 min) would fit well with the expected biochemical changes occurring in acute intermittent porphyria, indicating that this may be a suitable animal model for this disease.
研究了常用挥发性麻醉剂异氟烷对肝脏、肾脏和血液中某些血红素酶活性以及肝脏和血液中葡萄糖含量的影响。给小鼠注射不同剂量的该药物（0.5 - 6 mL/kg），并在注射后不同时间间隔（5 - 240分钟）处死。在此剂量范围内，2 mL/kg时对血红素代谢改变的效果最佳。每种酶的时间 - 反应曲线各不相同。麻醉后20分钟，血卟啉原酶（PBGase）和脱氨酶活性降低。这种降低与麻醉后不久（5分钟）观察到的δ-氨基-γ-酮戊酸合成酶活性诱导相结合，与急性间歇性卟啉病中预期发生的生化变化非常吻合，表明这可能是该疾病的合适动物模型。
