Styriak I, Nemcová R, Chang Y-H, Ljungh A
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia.
Lett Appl Microbiol. 2003;37(4):329-33. doi: 10.1046/j.1472-765x.2003.01402.x.
The aim of this study was to investigate extracellular matrix (ECM) and mucin binding of selected bacterial isolates with probiotic features in comparison with commercially used probiotic bacteria.
ECM molecules were immobilized in microtitre plates (mucin and fetuin) or on the surface of latex beads. Porcine mucin was bound by all 13 probiotic strains tested with important inter-strain differences; however, fetuin binding was similar (weak) for all 14 strains tested. Strongly positive (three) binding of bovine fibrinogen was expressed by strains from fermented food (Lactobacillus rhamnosus GG, L. casei Shirota and L. johnsonii La1) as well as by L. casei L.c., Lactobacillus sp. 2I3 and by L. plantarum LP. The other strains expressed moderate (2) or weakly positive (1) binding of bovine fibrinogen. Strongly positive (3) binding of porcine fibronectin was observed only with two strains; however, all other strains also bound this molecule. Bovine lactoferrin was bound to a higher extent than transferrins.
Some animal strains (at least L. casei L.c. and Lactobacillus sp. 2I3) are comparable with the commercially used strains with respect to their ECM binding ability. As this feature is important for probiotic bacteria to be able to colonize intestine, these strains should be considered for their wider use in fermented feed (or probiotic preparations) for animals.
本研究旨在调查具有益生菌特性的特定细菌分离株与市售益生菌相比，其细胞外基质（ECM）和黏蛋白结合情况。
将ECM分子固定在微量滴定板（黏蛋白和胎球蛋白）中或乳胶珠表面。所有13株受试益生菌菌株均能结合猪黏蛋白，且菌株间存在重要差异；然而，所有14株受试菌株对胎球蛋白的结合情况相似（较弱）。来自发酵食品的菌株（鼠李糖乳杆菌GG、干酪乳杆菌Shirota和约氏乳杆菌La1）以及干酪乳杆菌L.c.、乳杆菌属2I3和植物乳杆菌LP对牛纤维蛋白原表现出强阳性（3株）结合。其他菌株对牛纤维蛋白原表现出中度（2株）或弱阳性（1株）结合。仅观察到两株菌株对猪纤连蛋白有强阳性（3株）结合；然而，所有其他菌株也能结合该分子。牛乳铁蛋白的结合程度高于转铁蛋白。
一些动物源菌株（至少干酪乳杆菌L.c.和乳杆菌属2I3）在ECM结合能力方面与市售菌株相当。由于这一特性对于益生菌在肠道定殖很重要，因此应考虑将这些菌株更广泛地用于动物发酵饲料（或益生菌制剂）中。
