Sakata Keiko, Hirose Yoshinobu, Qiao Zheng, Tanaka Takuji, Mori Hideki
Department of Tumor Pathology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
Cancer Lett. 2003 Sep 25;199(2):139-45. doi: 10.1016/s0304-3835(03)00386-0.
Previous data have shown that a citrus flavanone, hesperidin possesses chemopreventive efficacies. We designed to investigate the inhibitory effect of hesperidin on lipopolysaccharide (LPS)-induced over-expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) proteins, over-production of prostaglandin E2 (PGE2) and nitric oxide (NO) using mouse macrophage cells. Treatment with hesperidin suppressed production of PGE2, nitrogen dioxide (NO2), and expression of iNOS protein. In the case of COX-2, hesperidin did not affect the protein level. Our data indicate hesperidin as a COX-2 and iNOS inhibitor, which might be related to the anti-inflammatory and anti-tumorigenic efficacies.
先前的数据表明，一种柑橘类黄酮橙皮苷具有化学预防功效。我们旨在研究橙皮苷对脂多糖（LPS）诱导的环氧化酶-2（COX-2）、诱导型一氧化氮合酶（iNOS）蛋白过度表达，以及前列腺素E2（PGE2）和一氧化氮（NO）过量产生的抑制作用，使用小鼠巨噬细胞进行研究。用橙皮苷处理可抑制PGE2、二氧化氮（NO2）的产生以及iNOS蛋白的表达。对于COX-2，橙皮苷不影响其蛋白水平。我们的数据表明橙皮苷是一种COX-2和iNOS抑制剂，这可能与其抗炎和抗肿瘤功效有关。
