Sun Junting, Yishake Hadir, Wang Ming, Zhang Hao, Yan Jie
College of Life Sciences, Shihezi University, Shihezi 832000, China.
Xinjiang Academy of Forestry Sciences, Urumqi 830000, China.
Biology (Basel). 2025 Feb 11;14(2):184. doi: 10.3390/biology14020184.
Figs are an edible and medicinal plant rich in polyphenols and flavonoids with unique pharmacological effects. However, the mechanism of flavonoid synthesis in figs is not clear. In this study, fig fruits of six varieties were collected for RNA sequencing and UPLC-MS data collection. The results showed that a total of 39 differential metabolites were identified by targeted metabolomics, and their contents were determined by UPLC-MS. The clustered heat map analysis showed that most of the differential metabolites were highly accumulated in BRD and FY. A total of 62 flavonoid biosynthesis pathway genes were identified by transcriptome analysis, and , , , , and were the key genes identified for the accumulation of flavonoids and flavonols in the dark-colored varieties. In addition, a total of 1671 transcription factor genes, mainly MYBs, bHLHs, and AP2/ERFs, were identified. This study will enrich the transcriptomic data of figs and provide some help in resolving the synthesis mechanism of fig flavonoids.
无花果是一种富含多酚和黄酮类化合物的可食用药用植物，具有独特的药理作用。然而，无花果中黄酮类化合物的合成机制尚不清楚。本研究收集了六个品种的无花果果实用于RNA测序和UPLC-MS数据采集。结果表明，通过靶向代谢组学共鉴定出39种差异代谢物，并用UPLC-MS测定了它们的含量。聚类热图分析表明，大多数差异代谢物在BRD和FY中高度积累。通过转录组分析共鉴定出62个黄酮类生物合成途径基因，其中 、 、 、 、 和 是深色品种中黄酮类和黄酮醇积累的关键基因。此外，共鉴定出1671个转录因子基因，主要是MYBs、bHLHs和AP2/ERFs。本研究将丰富无花果的转录组数据，并为解析无花果黄酮类化合物的合成机制提供一定帮助。
