Strecker Thomas, Eichler Robert, Meulen Jan ter, Weissenhorn Winfried, Dieter Klenk Hans, Garten Wolfgang, Lenz Oliver
Institut für Virologie der Philipps-Universität Marburg, D-35037 Marburg, Germany.
J Virol. 2003 Oct;77(19):10700-5. doi: 10.1128/jvi.77.19.10700-10705.2003.
Lassa virus is an enveloped virus with glycoprotein spikes on its surface. It contains an RNA ambisense genome that encodes the glycoprotein precursor GP-C, the nucleoprotein NP, the polymerase L, and the Z protein. Here we demonstrate that the Lassa virus Z protein (i). is abundant in viral particles, (ii). is strongly membrane associated, (iii). is sufficient in the absence of all other viral proteins to release enveloped particles, and (iv). contains two late domains, PTAP and PPXY, necessary for the release of virus-like particles. Our data provide evidence that Z is the Lassa virus matrix protein that is the driving force for virus particle release.
拉沙病毒是一种表面带有糖蛋白刺突的包膜病毒。它含有一个RNA双义基因组，该基因组编码糖蛋白前体GP-C、核蛋白NP、聚合酶L和Z蛋白。在此我们证明，拉沙病毒Z蛋白（i）. 在病毒颗粒中含量丰富，（ii）. 与膜紧密结合，（iii）. 在没有所有其他病毒蛋白的情况下足以释放包膜颗粒，以及（iv）. 包含两个晚期结构域PTAP和PPXY，这是释放病毒样颗粒所必需的。我们的数据提供了证据，表明Z是拉沙病毒的基质蛋白，是病毒颗粒释放的驱动力。
