从家蚕中分离出的一种脂肪酶对核型多角体病毒具有抗病毒活性。
A lipase isolated from the silkworm Bombyx mori shows antiviral activity against nucleopolyhedrovirus.
作者信息
Ponnuvel Kangayam M, Nakazawa Hiroshi, Furukawa Seiichi, Asaoka Ai, Ishibashi Jun, Tanaka Hiromitsu, Yamakawa Minoru
机构信息
Innate Immunity Laboratory, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8634, Japan.
出版信息
J Virol. 2003 Oct;77(19):10725-9. doi: 10.1128/jvi.77.19.10725-10729.2003.
Abstract
A protein showing strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) was purified from the digestive juice of B. mori larvae. A homology search of the deduced amino acid sequence of the protein cDNA revealed 56% homology with Drosophila melanogaster lipase and 21% homology with human lipase. As lipase activity of the protein was confirmed in vitro, this protein was designated Bmlipase-1. Northern blot analysis showed that the Bmlipase-1 gene is expressed in the midgut but not in other tissues, nor is it activated by BmNPV infection. In addition, the Bmlipase-1 gene was shown not to be expressed in the molting and wandering stages, indicating that the gene is hormonally regulated. Our results suggest that an insect digestive enzyme has potential as a physiological barrier against BmNPV at the initial site of viral infection.
摘要
从家蚕幼虫的消化液中纯化出一种对家蚕核型多角体病毒（BmNPV）具有强抗病毒活性的蛋白质。对该蛋白质cDNA推导的氨基酸序列进行同源性搜索发现，它与黑腹果蝇脂肪酶有56%的同源性，与人类脂肪酶有21%的同源性。由于该蛋白质的脂肪酶活性在体外得到证实，因此将其命名为Bmlipase-1。Northern印迹分析表明，Bmlipase-1基因在中肠表达，但在其他组织中不表达，也不会被BmNPV感染激活。此外，Bmlipase-1基因在蜕皮期和徘徊期不表达，这表明该基因受激素调节。我们的结果表明，一种昆虫消化酶在病毒感染的初始部位有可能作为对抗BmNPV的生理屏障。
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