氯化钆与丹参复方改善肝细胞线粒体的再灌注损伤。

Gadolinium chloride and salvia miltiorrhiza compound ameliorate reperfusion injury in hepatocellular mitochondria.

作者信息

Zhang Wen-Hai, Wang Jin-Sheng, Zhou Yong, Li Jian-Yi

机构信息

Department of General Surgery, Second Affiliated Hospital, China Medical University, 39 Huaxiang Road, Tiexi District, Shenyang 110022, Liaoning Province, China.

出版信息

World J Gastroenterol. 2003 Sep;9(9):2040-4. doi: 10.3748/wjg.v9.i9.2040.

DOI:10.3748/wjg.v9.i9.2040

PMID:12970902

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4656670/

Abstract

AIM

To investigate the effect of gadolinium chloride (GaCl(3)) and salvia miltiorrhiza compound (SMCo) on ischemia and reperfusion (I/R) injury in hepatocellular mitochondria.

METHODS

Wistar rats were randomly to divided into control group, GaCl(3) group, SMCo group and GaCl(3) + SMCo group (n=15 each). GaCl(3) (7 mg x kg(-1)) was injected into tail vein on d 1 and d 2 in contrast group. SMCo (2 ml x kg(-1)) was injected into muscle on d 1 and d 2 in SMCo group. GaCl(3)+SMCo group received both GaCl(3) (iv) and SMCo (im) injection. Control group received saline injection only. On d 3, all the rats were subjected to 2 h ischemia in the middle and left lobes of the liver, followed by reperfusion for 2 h, 6 h and 18 h respectively. The level of serum alanine aminotransferase (ALT) and malondialdehyde (MDA) in hepatocellular mitochondria was measured. Pathological changes in hepatic tissue and in hepatocellular mitochondria were determined with optical microscope and electronic microscope,respectively.

RESULTS

Remarkablly pathohistological and biochemical changes were detected after 6 h of I/R. Compared with control, the level of ALT was decreased in GaCl(3), SMCo and GaCl(3)+SMCo treated groups (1,314.0+/-278.7 vs 809.4+/-196.1, 716.6+/-242.8 and 837.2+/-190.6 IU x L(-1), respectively. P<0.05). Similarly, the level of MDA was decreased in GaCl(3), SMCo and GaCl(3)+SMCo treated groups (293.1+/-51.1 vs 190.8+/-55.5, 214.3+/-32.9 and 221.0+/-47.3 nmol x g(-1), respectively, P<0.05). Accordingly, in control group, swelling, degeneration, focal necrosis, infiltration of leucocyte were found in reperfused tissue under an optical microscope, and mitochondria swelling, rupture and even breakdown were seen under an electronic microscope. These pathohistological and ultrastructural damages caused by I/R were greatly attenuated in GaCl(3), SMCo and GaCl(3)+SMCo treated groups. However, there was no additive effect observed when GaCl(3) and SMCo were used together.

CONCLUSION

Both GaCl(3) and SMCo can alleviate the I/R injury in hepatocellular mitochondria.

摘要

目的

探讨氯化钆（GaCl₃）和复方丹参（SMCo）对肝细胞线粒体缺血再灌注（I/R）损伤的影响。

方法

将Wistar大鼠随机分为对照组、GaCl₃组、SMCo组和GaCl₃+SMCo组（每组n = 15）。对照组于第1天和第2天经尾静脉注射GaCl₃（7 mg·kg⁻¹）。SMCo组于第1天和第2天经肌肉注射SMCo（2 ml·kg⁻¹）。GaCl₃+SMCo组同时接受GaCl₃静脉注射和SMCo肌肉注射。对照组仅注射生理盐水。第3天，所有大鼠肝脏中叶和左叶进行2 h缺血，随后分别再灌注2 h、6 h和18 h。检测肝细胞线粒体中血清丙氨酸氨基转移酶（ALT）和丙二醛（MDA）水平。分别用光学显微镜和电子显微镜观察肝组织和肝细胞线粒体的病理变化。

结果

I/R 6 h后检测到明显的病理组织学和生化变化。与对照组相比，GaCl₃组、SMCo组和GaCl₃+SMCo组的ALT水平降低（分别为1,314.0±278.7 vs 809.4±196.1、716.6±242.8和837.2±190.6 IU·L⁻¹，P<0.05）。同样，GaCl₃组、SMCo组和GaCl₃+SMCo组的MDA水平降低（分别为293.1±51.1 vs 190.8±55.5、214.3±32.9和221.0±47.3 nmol·g⁻¹，P<0.05）。因此，对照组在光学显微镜下可见再灌注组织中有肿胀、变性、局灶性坏死、白细胞浸润，在电子显微镜下可见线粒体肿胀、破裂甚至崩解。I/R引起的这些病理组织学和超微结构损伤在GaCl₃组、SMCo组和GaCl₃+SMCo组中明显减轻。然而，GaCl₃和SMCo联合使用时未观察到相加效应。