Gao Mengsheng, Teplitski Max, Robinson Jayne B, Bauer Wolfgang D
Department of Horticulture and Crop Science, 2021 Coffey Rd., Ohio State University, Columbus, OH 43210, USA.
Mol Plant Microbe Interact. 2003 Sep;16(9):827-34. doi: 10.1094/MPMI.2003.16.9.827.
Earlier work showed that higher plants produce unidentified compounds that specifically stimulate or inhibit quorum sensing (QS) regulated responses in bacteria. The ability of plants to produce substances that affect QS regulation may provide plants with important tools to manipulate gene expression and behavior in the bacteria they encounter. In order to examine the kinds of QS active substances produced by the model legume M. truncatula, young seedlings and seedling exudates were systematically extracted with various organic solvents, and the extracts were fractionated by reverse phase C18 high-performance liquid chromatography. M. truncatula appears to produce at least 15 to 20 separable substances capable of specifically stimulating or inhibiting responses in QS reporter bacteria, primarily substances that affect QS regulation dependent on N-acyl homoserine lactone (AHL) signals. The secretion of AHL QS mimic activities by germinating seeds and seedlings was found to change substantially with developmental age. The secretion of some mimic activities may be dependent upon prior exposure of the plants to bacteria.
早期研究表明，高等植物会产生一些未知化合物，这些化合物能特异性地刺激或抑制细菌群体感应（QS）调控的反应。植物产生影响QS调控物质的能力，可能为植物提供了重要工具，用以操控它们所接触细菌中的基因表达和行为。为了研究模式豆科植物截形苜蓿产生的QS活性物质种类，用各种有机溶剂对幼苗和幼苗渗出物进行了系统提取，提取物通过反相C18高效液相色谱进行分离。截形苜蓿似乎能产生至少15至20种可分离的物质，这些物质能够特异性地刺激或抑制QS报告菌的反应，主要是影响依赖N-酰基高丝氨酸内酯（AHL）信号的QS调控的物质。研究发现，发芽种子和幼苗分泌的AHL QS模拟活性会随着发育年龄发生显著变化。一些模拟活性的分泌可能取决于植物之前与细菌的接触。
