Paulík S, Svrcek S, Húska M, Mojzisová J, Durove A, Benísek Z
Univerzita veterinárskeho lekárstva, Kosice.
Vet Med (Praha). 1992 Dec;37(12):675-85.
The level of cell-mediated immune response in vivo was investigated using the test of delayed hypersensitive reaction (DHR) to DNFB, along with the phagocytic activity (PA) of blood leucocytes in mice after subcutaneous implantation of fungal and yeast glucan and levamisol in dependence on the dose and administration schedule. The soluble form of fungal glucan (Pleurotus ostreatus) potentiated the DHR significantly at a dose of 10 mg/kg (but not at a dose of 50 mg/kg) while it was administered during DNFB sensitization (P < 0.05)-Tab. I and when its pre-medication effect was investigated (days -7 and -14; P < 0.05) with regard to the time of sensitization (Tab. II). The identical dose of glucan also had a positive effect (P < 0.05 or 0.01) on the percentual proportion of phagocytic cells (PC) reaching the maximum in the 2nd and 3rd week of investigation, as well as on the phagocytic activity index (P < 0.05; 3rd week) and percentage of neutrophil granulocytes (P < 0.05; 2nd week)-Tab. III. Yeast glucan (Saccharomyces cerevisiae) showed a potentiating effect on the DHR to DNFB only in the case of its pre-medication use; its soluble form was effective at both doses (10 mg and 50 mg/kg) in days -7 and -14 (P < 0.05), and its corpuscular form at a dose of 50 mg/kg on days -7, -14 and -21 (P < 0.05 or 0.01)-Tab. II. PA parameters of blood leucocytes displayed a stimulative effect only on the PC percentage. The most significant effects in this case were observed in the soluble form (both doses) in the 2nd and 3rd week (P < 0.01 and 0.05, resp.) and in the insoluble form (both doses) in the 3rd and 4th week of observation (P < 0.05 and 0.01, resp.). An increase in the number of neutrophil granulocytes was significant in the 2nd (P < 0.05 or 0.01; corpuscular form) and 3rd week of the experiment (P < 0.01; soluble form)-Tab. III. Levamisol affected both investigated parameters (DHR and PA) only at a dose 20 mg/kg (10 mg/kg-no effect). Its potentiating effect on the DHR level was observed both for its administration at the time of sensitization (P < 0.05) and for its administration on days 7 (P < 0.05) and 14 (P < 0.01) before DNFB sensitization (Tabs. I and II). A statistically significant increase in PC was recorded in weeks 2, 3 and 4 (P < 0.05 or 0.01), a statistically significant increase in the number of neutrophil granulocytes in the 3rd week of investigation (P < 0.05). The phagocytic activity index was not affected.
通过对二硝基氟苯（DNFB）的迟发型超敏反应（DHR）试验，研究了体内细胞介导的免疫反应水平，同时还研究了在小鼠皮下植入真菌和酵母葡聚糖以及左旋咪唑后，其剂量和给药方案对血液白细胞吞噬活性（PA）的影响。真菌葡聚糖（平菇）的可溶形式在剂量为10mg/kg时（但50mg/kg剂量时无此效果），在DNFB致敏期间给药，可显著增强DHR（P<0.05）（表I），并且在研究致敏时间时（表II），在-7天和-14天进行预处理时也有显著增强作用（P<0.05）。相同剂量的葡聚糖对吞噬细胞（PC）百分比也有积极影响（P<0.05或0.01），在研究的第2周和第3周达到最大值，对吞噬活性指数也有影响（P<0.05；第3周），对中性粒细胞百分比也有影响（P<0.05；第2周）（表III）。酵母葡聚糖（酿酒酵母）仅在预处理时对DNFB的DHR有增强作用；其可溶形式在-7天和-14天的两种剂量（10mg和50mg/kg）下均有效（P<0.05），其颗粒形式在-7天、-14天和-21天剂量为50mg/kg时有效（P<0.05或0.01）（表II）。血液白细胞的PA参数仅对PC百分比有刺激作用。在这种情况下，最显著的影响在第2周和第3周的可溶形式（两种剂量）中观察到（分别为P<0.01和0.05），在观察的第3周和第4周的不溶形式（两种剂量）中观察到（分别为P<0.05和0.01）。中性粒细胞数量的增加在实验的第2周（P<0.05或0.01；颗粒形式）和第3周（P<0.01；可溶形式）显著（表III）。左旋咪唑仅在剂量为20mg/kg时影响两个研究参数（DHR和PA）（10mg/kg无影响）。在致敏时给药（P<0.05）以及在DNFB致敏前第7天（P<0.05）和第14天（P<0.01）给药时，均可观察到其对DHR水平的增强作用（表I和表II）。在第2、3和4周记录到PC有统计学显著增加（P<0.05或0.01），在研究的第3周中性粒细胞数量有统计学显著增加（P<0.05）。吞噬活性指数未受影响。
