无体腔人类寄生虫曼氏血吸虫的转录组分析。

Transcriptome analysis of the acoelomate human parasite Schistosoma mansoni.

作者信息

Verjovski-Almeida Sergio, DeMarco Ricardo, Martins Elizabeth A L, Guimarães Pedro E M, Ojopi Elida P B, Paquola Apuã C M, Piazza João P, Nishiyama Milton Y, Kitajima João P, Adamson Rachel E, Ashton Peter D, Bonaldo Maria F, Coulson Patricia S, Dillon Gary P, Farias Leonardo P, Gregorio Sheila P, Ho Paulo L, Leite Ricardo A, Malaquias L Cosme C, Marques Regina C P, Miyasato Patricia A, Nascimento Ana L T O, Ohlweiler Fernanda P, Reis Eduardo M, Ribeiro Marcela A, Sá Renata G, Stukart Gaëlle C, Soares M Bento, Gargioni Cybele, Kawano Toshie, Rodrigues Vanderlei, Madeira Alda M B N, Wilson R Alan, Menck Carlos F M, Setubal João C, Leite Luciana C C, Dias-Neto Emmanuel

机构信息

Departamento de Bioquimica, Instituto de Quimica, Universidade de São Paulo, 05508-900 São Paulo, SP, Brazil.

出版信息

Nat Genet. 2003 Oct;35(2):148-57. doi: 10.1038/ng1237. Epub 2003 Sep 14.

DOI:10.1038/ng1237

PMID:12973350

Abstract

Schistosoma mansoni is the primary causative agent of schistosomiasis, which affects 200 million individuals in 74 countries. We generated 163,000 expressed-sequence tags (ESTs) from normalized cDNA libraries from six selected developmental stages of the parasite, resulting in 31,000 assembled sequences and 92% sampling of an estimated 14,000 gene complement. By analyzing automated Gene Ontology assignments, we provide a detailed view of important S. mansoni biological systems, including characterization of metazoa-specific and eukarya-conserved genes. Phylogenetic analysis suggests an early divergence from other metazoa. The data set provides insights into the molecular mechanisms of tissue organization, development, signaling, sexual dimorphism, host interactions and immune evasion and identifies novel proteins to be investigated as vaccine candidates and potential drug targets.

摘要

曼氏血吸虫是血吸虫病的主要病原体，该病在74个国家影响着2亿人。我们从该寄生虫六个选定发育阶段的标准化cDNA文库中生成了163,000个表达序列标签（EST），得到了31,000个组装序列，对估计的14,000个基因互补体的采样率达到92%。通过分析自动基因本体分配，我们详细了解了重要的曼氏血吸虫生物系统，包括后生动物特异性和真核生物保守基因的特征。系统发育分析表明它与其他后生动物早期分化。该数据集为组织组织、发育、信号传导、两性异形、宿主相互作用和免疫逃避的分子机制提供了见解，并鉴定出作为疫苗候选物和潜在药物靶点进行研究的新蛋白质。