Molina J M, Córdoba J, Monsoliu A, Diosdado N, Gobernado M
Servicio de Microbiología, Hospital Universitario La Fe, Valencia, Spain.
Rev Esp Quimioter. 2003 Jun;16(2):195-203.
The resistance to betalactam antibiotics in a total of 177 clinical isolates of Haemophilus influenzae, mostly from respiratory tract samples and characterized by their betalactamase production, was studied using the cephalosporin chromogenic assay and by detecting bla(TEM) and bla(ROB) genes. A substantial number of clinical isolates carrying the bla(TEM) gene, which presented a mutation consisting of the absence of a fragment of 136 base pairs, located upstream from the coding region including the 35 but not the 10 region of the promoter were found. This suggests that a new bla(TEM) promoter exists in these strains. This finding was associated with increased resistance to the antibiotics cefaclor and loracarbef compared with normal isolates. It was also found that 3.9% of the isolates carried the bla(ROB-1) gene.
对总共177株流感嗜血杆菌临床分离株（大多来自呼吸道样本，并根据其β-内酰胺酶产生情况进行了表征）对β-内酰胺类抗生素的耐药性进行了研究，采用头孢菌素显色测定法并检测bla(TEM)和bla(ROB)基因。发现大量携带bla(TEM)基因的临床分离株存在一种突变，该突变表现为在编码区上游缺失136个碱基对的片段，该编码区包括启动子的35区域但不包括10区域。这表明这些菌株中存在一种新的bla(TEM)启动子。与正常分离株相比，这一发现与对头孢克洛和氯碳头孢抗生素的耐药性增加有关。还发现3.9%的分离株携带bla(ROB-1)基因。
