流感嗜血杆菌中blaTEM基因及启动子特定区域的变异
Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae.
作者信息
Tristram Stephen G, Hawes Rebecca, Souprounov Juliana
机构信息
School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia.
出版信息
J Antimicrob Chemother. 2005 Sep;56(3):481-4. doi: 10.1093/jac/dki238. Epub 2005 Jul 8.
OBJECTIVES
To determine if the TEM beta-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.
METHODS
Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the bla(TEM) genes of a collection of TEM-positive strains.
RESULTS
All bla(TEM) genes were found to be bla(TEM-1) or derivatives thereof and none bla(TEM-2). The bla(TEM) genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.
CONCLUSIONS
The genetic features of bla(TEM) genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.
目的
确定流感嗜血杆菌的 TEM β-内酰胺酶是否为 TEM-1 或其衍生物，以及是否仅与重叠的 Pa/Pb 启动子相关。
方法
使用单核苷酸特异性 PCR 鉴别一组 TEM 阳性菌株 bla(TEM)基因第 32 和 317 位的多态性核苷酸。
结果
所有 bla(TEM)基因均为 bla(TEM-1)或其衍生物，无 bla(TEM-2)。bla(TEM)基因与 P3 启动子、Pa/Pb 启动子或因 135 bp 缺失和 G162T 取代而产生的新型启动子相关。
结论
流感嗜血杆菌中 bla(TEM)基因的遗传特征不同于肠杆菌科，且比之前认识到的更具变异性。
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