Al-Arfaj Abdurhman S, Mustafa Ali A, Alballa Suliman R, Tuwaijri Ali S, Al-Dalaan Abdullah N
Department of Medicine, College of Medicine, King Saud University, Riyadh, Kingdom of Saudi ARabia.
Saudi Med J. 2003 Sep;24(9):936-40.
To find out the effect of combining allopurinol with non-steroidal anti-inflammatory drugs on carrageenan-induced rat paw edema.
The study was carried out at the College of Medicine, King Saud University, Riyadh, Kingdom of Saudi Arabia, over the period 1999 to 2000. Male wistar rats were randomly divided into 12-16 rats in each group. Edema was induced by subplantar injection of 0.1 ml of carrageenan (10 mg/ml) and the resulting edema volume was measured by plethysmograph, 3 hours after the injections. Saline of 0.9% (0.1 ml/100 g) was administered to the first group serving as control. The second and third groups received variable concentration of allopurinol (12.5, 25, 50 mg/kg) and tenoxicam (0.0625, 0.125, 0.25 mg/kg) 30 minutes before carrageenan injection. The fourth group received a combination of tenoxicam and allopurinol. Similar procedures were carried out with respect to diclofenac at 1.25, 2.5, 5.0 mg/kg and indomethacin at 0.25, 0.5, 1.0 mg/kg. The activities of the drugs were expressed as percentage inhibition of edema.
Pre-treatment of the rats with the 4 drugs individually resulted in dose-dependent reduction of volume of paw edema. The combination of allopurinol and diclofenac acted synergistically to reduce edema. A similar synergistic action was obtained when allopurinol was combined with indomethacin. By contrast, tenoxicam-allopurinol combination resulted in antagonistic action and produced an effect on edema, which was less than their individual inhibitory action.
Combining allopurinol with either diclofenac or indomethacin produced synergistic inhibitory action on rat's paw edema. However, tenoxicam, when combined with allopurinol, produced antagonism.
探究别嘌醇与非甾体抗炎药联合使用对角叉菜胶诱导的大鼠足爪肿胀的影响。
该研究于1999年至2000年在沙特阿拉伯王国利雅得的沙特国王大学医学院进行。雄性Wistar大鼠被随机分为每组12 - 16只。通过足底注射0.1 ml角叉菜胶（10 mg/ml）诱导肿胀，注射3小时后用体积描记器测量由此产生的肿胀体积。第一组给予0.9%生理盐水（0.1 ml/100 g）作为对照。第二组和第三组在注射角叉菜胶前30分钟接受不同浓度的别嘌醇（12.5、25、50 mg/kg）和替诺昔康（0.0625、0.125、0.25 mg/kg）。第四组接受替诺昔康和别嘌醇的组合。对于双氯芬酸（1.25、2.5、5.0 mg/kg）和吲哚美辛（0.25、0.5、1.0 mg/kg）进行了类似的操作。药物活性以肿胀抑制百分比表示。
用这4种药物单独预处理大鼠导致足爪肿胀体积呈剂量依赖性减少。别嘌醇和双氯芬酸联合使用具有协同作用以减轻肿胀。当别嘌醇与吲哚美辛联合使用时也获得了类似的协同作用。相比之下，替诺昔康 - 别嘌醇组合产生拮抗作用，对肿胀产生的影响小于它们各自的抑制作用。
别嘌醇与双氯芬酸或吲哚美辛联合使用对大鼠足爪肿胀产生协同抑制作用。然而，替诺昔康与别嘌醇联合使用时产生拮抗作用。
