Silva Luciana Pereira, Barcelos Ivanildes Solange da Costa, Passos-Lima Andréia Barcelos, Espindola Foued Salmen, Campos Dulcinéa Maria Barbosa, Costa-Cruz Julia Maria
Departamento de Imunologia, Microbiologia e Parasitologia, Instituto de Ciências Biomédicas, Uberlândia, Brasil.
Mem Inst Oswaldo Cruz. 2003 Jul;98(5):687-91. doi: 10.1590/s0074-02762003000500017. Epub 2003 Sep 8.
The present study was conducted to evaluate the frequency of antigenic components recognized by serum IgG antibodies in Western blotting (WB) using a Strongyloides ratti larval extract for the diagnosis of human strongyloidiasis. In addition, the WB results were compared to the enzyme-linked immunosorbent assay (ELISA) and the indirect immunofluorescence antibody test (IFAT) results. Serum samples of 180 individuals were analyzed (80 with strongyloidiasis, 60 with other intestinal parasitoses, and 40 healthy individuals). S. ratti was obtained from fecal culture of experimentally infected Rattus rattus. For IFAT, S. ratti larvae were used as antigen and S. ratti larval antigenic extracts were employed in WB and ELISA. Eleven S. ratti antigenic components were predominantly recognized by IgG antibodies in sera of patients with strongyloidiasis. There was a positive concordance for the three tests in 87.5% of the cases of strongyloidiasis. The negative concordance in the three tests was 94% and 97.5%, in patients with other intestinal parasitoses and healthy individuals, respectively. In cases of positive ELISA and negative IFAT results, diagnosis could be confirmed by WB. ELISA, IFAT, and WB using S. ratti antigens showed a high rate of sensitivity and specificity. In conclusion, WB using S. ratti larval extract was able to recognize 11 immunodominant antigenic components, showing to be a useful tool to define the diagnosis in cases of equivocal serology.
本研究旨在评估在蛋白质印迹法（WB）中，使用鼠类圆线虫幼虫提取物来诊断人类类圆线虫病时，血清IgG抗体识别的抗原成分频率。此外，还将WB结果与酶联免疫吸附测定（ELISA）和间接免疫荧光抗体试验（IFAT）结果进行了比较。分析了180名个体的血清样本（80名患有类圆线虫病，60名患有其他肠道寄生虫病，40名健康个体）。鼠类圆线虫取自实验感染的褐家鼠粪便培养物。对于IFAT，使用鼠类圆线虫幼虫作为抗原，而在WB和ELISA中使用鼠类圆线虫幼虫抗原提取物。11种鼠类圆线虫抗原成分主要被类圆线虫病患者血清中的IgG抗体识别。在87.5%的类圆线虫病病例中，这三种检测方法呈阳性一致性。在患有其他肠道寄生虫病的患者和健康个体中，这三种检测方法的阴性一致性分别为94%和97.5%。在ELISA结果为阳性而IFAT结果为阴性的情况下，可通过WB确诊。使用鼠类圆线虫抗原的ELISA、IFAT和WB显示出高灵敏度和特异性。总之，使用鼠类圆线虫幼虫提取物的WB能够识别11种免疫显性抗原成分，表明它是在血清学结果不明确的情况下进行诊断的有用工具。
