Nageswaran C, Craig P S, Devaney E
Department of Parasitology, Liverpool School of Tropical Medicine.
Parasitology. 1994 Apr;108 ( Pt 3):335-42. doi: 10.1017/s0031182000076174.
The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens. The first detection of coproantigen coincided with patency and the coproantigen level remained elevated even when the parasite count was low. Following challenge infection, when examination of stool for larvae or ova was negative, coproantigen was detected by anti-adult somatic capture ELISA. Despite the use of antisera raised against crude worm extracts, the test was specific showing only low cross-reactivity with Necator americanus and Syphacia muris.
在实验感染鼠类圆线虫的大鼠中，研究了粪抗原检测在类圆线虫病诊断中的可能性。利用在兔体内产生的针对成虫和丝状幼虫体抗原的亲和纯化多克隆抗体制备了一种捕获ELISA。粪抗原的首次检测与虫体出现巧合，即使寄生虫数量较低时，粪抗原水平仍保持升高。再次感染后，当粪便幼虫或虫卵检查为阴性时，通过抗成虫体捕获ELISA检测到粪抗原。尽管使用了针对粗制虫体提取物产生的抗血清，但该检测具有特异性，仅与美洲钩虫和鼠管状线虫有低交叉反应性。
