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香蕉农业废弃物的利用:土壤真菌生产纤维素酶

Utilization of banana agricultural waste: production of cellulases by soil fungi.

作者信息

Baig M M V, Mane V P, More D R, Shinde L P, Baig M I A

机构信息

Botany Research Laboratory & Plant Disease Clinic, Science College, Nanded-431605, Maharashtra, India.

出版信息

J Environ Biol. 2003 Apr;24(2):173-6.

Abstract

Banana a major cash crop of Maharashtra is cultivated over 46900 hectares generating large amount of agro waste after the harvest. Attempts were made to utilize these agro wastes for production of cellulases. Of the 127 fungi isolated from the soil of banana field, 12 fungi were found to utilize cellulose as source of carbon. Trichoderma lignorum showed appreciable cellulolytic activity. It produced Cl, Cx and beta glucosidase in Carboxymethyl Cellulose Peptone medium as well as on agro waste based medium containing leaves, stem and rhizome powders. T. lignorum (0. 45 U/ml) produced maximum enzymes on leaf based medium.

摘要

香蕉是马哈拉施特拉邦的主要经济作物,种植面积超过46900公顷,收获后会产生大量农业废弃物。人们尝试利用这些农业废弃物来生产纤维素酶。从香蕉园土壤中分离出的127种真菌中,有12种被发现能够利用纤维素作为碳源。木素木霉表现出明显的纤维素分解活性。它在羧甲基纤维素蛋白胨培养基以及含有叶片、茎和根茎粉末的农业废弃物培养基上都能产生内切葡聚糖酶、外切葡聚糖酶和β-葡萄糖苷酶。木素木霉在以叶片为基础的培养基上产生的酶量最多(0.45 U/ml)。

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