Gupta J K, Shirkot C K, Dhawan S
Acta Microbiol Acad Sci Hung. 1981;28(1):31-6.
Nineteen fungi were isolated from different soil samples on the basis of clear zones formed on Rose Bengal Cellulose agar medium. In shake flasks th isolate K1 gave 12.1 units/ml of CMCase activity. A mutant of the isolate K1, KM7, was selected after N-methyl-N'-nitro-N-nitrosoguanidine treatment of the wild-type. This mutant differed morphologically from the parent strain on RBCA medium and gave 36.2 units/ml of CMCase activity which represented about 50% of the enzyme yield from the standard organism, Trichoderma viride QM 9414 (80 units/ml of CMCase activity). The isolate K1, which was identified as a Phoma species, produced 48 units of beta-glucosidase. The yield of beta-glucosidase was increased about 8-fold in the mutant KM7 and was about 68% higher than the level found in T. viride QM 9414.
基于孟加拉红纤维素琼脂培养基上形成的透明圈，从不同土壤样品中分离出19种真菌。在摇瓶中，分离株K1产生12.1单位/毫升的羧甲基纤维素酶（CMCase）活性。对野生型进行N-甲基-N'-硝基-N-亚硝基胍处理后，筛选出分离株K1的一个突变体KM7。该突变体在孟加拉红纤维素琼脂（RBCA）培养基上的形态与亲本菌株不同，产生36.2单位/毫升的CMCase活性，约占标准菌株绿色木霉QM 9414（80单位/毫升的CMCase活性）酶产量的50%。被鉴定为茎点霉属物种的分离株K1产生48单位的β-葡萄糖苷酶。突变体KM7中β-葡萄糖苷酶的产量增加了约8倍，比绿色木霉QM 9414中的水平高约68%。
