Kamitani Y, Kajii E, Suda T, Ikemoto S
Department of Legal Medicine and Human Genetics, Jichi Medical School, Tochigi, Japan.
Jpn J Hum Genet. 1992 Dec;37(4):271-83. doi: 10.1007/BF01883318.
In order to examine expression of the Tn antigen on erythroid cells from a patient with Tn syndrome, we applied a selective two phase liquid culture system for human erythroid progenitors in peripheral blood. The cells were analyzed with flow cytometry employing an anti-Tn antibody and a lectin of Vicia villosa which recognizes only the Tn determinant. In the second phase, the Tn antigen was expressed on the cultured cells from the patient on day 3 and Tn-positive cells reached 62.7% on day 9. On the other hand, Tn-positive cells were not detected in the volunteer's cultured cells. When the patient's cells were co-cultured with the cells from a healthy volunteer, the percentage of Tn-positive cells was much lower than the expected value, suggesting that the normal cells suppressed the expression of Tn antigen on the patient's cells.
为检测Tn综合征患者红系细胞上Tn抗原的表达，我们应用了一种针对人外周血红系祖细胞的选择性两相液体培养系统。使用抗Tn抗体和仅识别Tn决定簇的野豌豆凝集素，通过流式细胞术对细胞进行分析。在第二阶段，患者培养细胞在第3天表达Tn抗原，第9天Tn阳性细胞达到62.7%。另一方面，在志愿者的培养细胞中未检测到Tn阳性细胞。当患者细胞与健康志愿者的细胞共培养时，Tn阳性细胞的百分比远低于预期值，这表明正常细胞抑制了患者细胞上Tn抗原的表达。
