Sixou M
Department of Epidemiology, Dental School, Toulouse, France.
Oral Dis. 2003;9 Suppl 1:54-62. doi: 10.1034/j.1601-0825.9.s1.10.x.
The bacteriological tests in periodontology have been available to clinicians since the end of the 1980s. Despite the vast and varied information that they contribute to the literature on this topic, some controversy has arisen with regard to the use of these tests. Clinicians faced with a multitude of clinical forms of periodontal disease in their patients can count on laboratory examinations to help them in the therapeutic process. These biological examinations can offer help in five areas of application: support in diagnosis and prognosis, verification of the efficiency of the treatment, indication of the correct antibiotic therapy and choice of the most appropriate molecules. The clinician's needs determine the type of bacteriological technical analysis used: bacterial culture or genetic identification using the molecular techniques. Each technique has its advantages and disadvantages. Knowing these limits allows the most appropriate choice to be made, depending on the clinical situation. The richness of information provided by culture in the anti-microbial susceptibility test and the non-targeted aspect of this test make this technique very interesting for the diagnosis of periodontitis. However, the targeted aspect of molecular techniques (PCR), their excellent sensibility and their rapidity make them prevail, particularly in the control and maintenance phases. Furthermore, despite the constant development of bacterial identification techniques, these examinations are little used by dental surgeons as these examinations are little known. They are also difficult to interpret and generate heavy additional costs.
自20世纪80年代末以来,牙周病学中的细菌学检测已可供临床医生使用。尽管这些检测为该主题的文献提供了大量多样的信息,但在这些检测的使用方面仍出现了一些争议。面对患者多种临床形式牙周病的临床医生可以依靠实验室检查来帮助他们进行治疗过程。这些生物学检查可在五个应用领域提供帮助:支持诊断和预后、验证治疗效果、指明正确的抗生素治疗以及选择最合适的药物。临床医生的需求决定了所使用的细菌学技术分析类型:细菌培养或使用分子技术进行基因鉴定。每种技术都有其优缺点。了解这些局限性有助于根据临床情况做出最合适的选择。培养在抗菌药敏试验中提供的丰富信息以及该试验的非靶向性使得该技术对牙周炎的诊断非常有意义。然而,分子技术(PCR)的靶向性、出色的敏感性和快速性使其占优势,特别是在控制和维持阶段。此外,尽管细菌鉴定技术不断发展,但这些检查很少被牙科医生使用,因为这些检查鲜为人知。它们也难以解释且会产生大量额外费用。