肠道微生物和口腔假单胞菌中对四环素和β-内酰胺类的耐药性以及耐药标记物的分布。
Resistance to tetracycline and β-lactams and distribution of resistance markers in enteric microorganisms and pseudomonads isolated from the oral cavity.
机构信息
Department of Pathology and Clinical Propedeutics, School of Dentistry of Araçatuba, São Paulo State University.
出版信息
J Appl Oral Sci. 2009;17 Suppl(spe):13-8. doi: 10.1590/s1678-77572009000700004.
Abstract
This study evaluated the occurrence of enteric bacteria and pseudomonads resistant to tetracycline and β-lactams in the oral cavity of patients exhibiting gingivitis (n=89), periodontitis (n=79), periodontally healthy (n=50) and wearing complete dentures (n=41). Microbial identification and presence of resistance markers associated with the production of β-lactamases and tetracycline resistance were performed by using biochemical tests and PCR. Susceptibility tests were carried out in 201 isolates of enteric cocci and rods. Resistance to ampicillin, amoxicillin/clavulanic acid, imipenem, meropenem and tetracycline was detected in 57.4%, 34.6%, 2.4%, 1.9% and 36.5% of the isolates, respectively. β-lactamase production was observed in 41.2% of tested microorganisms, while the most commonly found β-lactamase genetic determinant was gene blaTEM. Tetracycline resistance was disseminated and a wide scope of tet genes were detected in all studied microbial genus.
摘要
本研究评估了患有牙龈炎（n=89）、牙周炎（n=79）、牙周健康（n=50）和佩戴全口义齿（n=41）的患者口腔中对四环素和β-内酰胺类药物具有耐药性的肠细菌和假单胞菌的发生情况。通过生化试验和 PCR 检测，对微生物鉴定和与β-内酰胺酶和四环素耐药性产生相关的耐药标记物进行了检测。对 201 株肠球菌和杆菌分离株进行了药敏试验。在检测到的分离株中，分别有 57.4%、34.6%、2.4%、1.9%和 36.5%对氨苄西林、阿莫西林/克拉维酸、亚胺培南、美罗培南和四环素耐药。41.2%的测试微生物产生了β-内酰胺酶，而最常见的β-内酰胺酶基因决定簇是 blaTEM 基因。四环素耐药性广泛传播，在所有研究的微生物属中都检测到了多种 tet 基因。
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